Svedmyr E, Jondal M, Leibold W
Scand J Immunol. 1975;4(7):721-34. doi: 10.1111/j.1365-3083.1975.tb02680.x.
Lymphocytes from normal adults, with or without serological signs of previous Epstein-Barr virus (EBV) infection, could be stimulated to proliferate and produce killer cells by incubation with autologous EBV-genome-positive lymphoid cell lines (LCLs). The stimulated cells were most probably of T-cell origin, although at the peak of stimulation many of them lacked the sheep erythrocyte marker. Direct effector-target cell contact was necessary for lysis to occur. The cytotoxicity of autologously stimulated (AS) lymphocytes was not restricted to EBV-genome-positive LCLs, nor to cell lines of hematopoietic origin. It was equally broad if cells carrying complement receptor had been removed before stimulation. Fresh lymphocytes, blasts induced by phytohemagglutinin or concanavalin A, and Burkitt's lymphoma biopsy cells were resistant or considerably less sensitive. Mouse cells--even cell lines--were resistant. The sensitivity of target cells to lysis correlated positively with their capacity to block AS lymphocyte lysis of autologous LCLs in competition experiments. The cytotoxicity of AS lymphocytes was blocked by EBV-genome-positive and -negative cell lines, whereas the EBV-related cytotoxicity of T cells from acute cases of infectious mononucleosis was blocked by EBV-genome-positive LCL only.
来自正常成年人的淋巴细胞，无论有无既往爱泼斯坦-巴尔病毒（EBV）感染的血清学迹象，与自体EBV基因组阳性淋巴母细胞系（LCLs）孵育后，均可被刺激增殖并产生杀伤细胞。尽管在刺激高峰期许多细胞缺乏绵羊红细胞标记，但被刺激的细胞很可能起源于T细胞。细胞毒性作用的发生需要效应细胞与靶细胞直接接触。自体刺激（AS）淋巴细胞的细胞毒性并不局限于EBV基因组阳性的LCLs，也不局限于造血来源的细胞系。如果在刺激前去除携带补体受体的细胞，其细胞毒性作用范围同样广泛。新鲜淋巴细胞、由植物血凝素或刀豆球蛋白A诱导的母细胞以及伯基特淋巴瘤活检细胞具有抗性或敏感性明显较低。小鼠细胞——甚至细胞系——也具有抗性。在竞争实验中，靶细胞对裂解的敏感性与其阻断AS淋巴细胞对自体LCLs裂解的能力呈正相关。AS淋巴细胞的细胞毒性被EBV基因组阳性和阴性细胞系所阻断，而传染性单核细胞增多症急性病例中T细胞的EBV相关细胞毒性仅被EBV基因组阳性LCLs所阻断。
