具有自然杀伤细胞特异性的细胞毒性效应细胞的体外诱导
In vitro induction of cytotoxic effector cells with spontaneous killer cell specificity.
作者信息
Jondal M, Targan S
出版信息
J Exp Med. 1978 Jun 1;147(6):1621-36. doi: 10.1084/jem.147.6.1621.
Abstract
The present work shows that stimulation of peripheral blood lymphocytes with autologous or allogeneic B-cell lines leads to a strong induction of cytotoxic effector cells with spontaneous killer (SK) cell specificity, apart from the specific response directed against the particular stimulating cell. To demonstrate this we have determined a relative target cell specificity in the SK system, defined by the short-term 51Cr release assay, and established a relative specificity index (RSI). Using this approach we have been able to show that killer cells induced during a 5-day cocultivation period with B-cell lines have a similar PSI to that of unstimulated SK cells. In addition, we have shown that such killer cells can be induced from several different lymphocyte subpopulations and that they, in contrast to SK cells, do not express Fc receptors. The implications of these findings in relation to the nature, mechanism, and biological significance of the SK cell system is discussed.
摘要
目前的研究表明，用自体或同种异体B细胞系刺激外周血淋巴细胞，除了针对特定刺激细胞的特异性反应外，还会强烈诱导具有自发杀伤（SK）细胞特异性的细胞毒性效应细胞。为了证明这一点，我们通过短期51Cr释放试验确定了SK系统中的相对靶细胞特异性，并建立了相对特异性指数（RSI）。使用这种方法，我们能够证明在与B细胞系共培养5天期间诱导的杀伤细胞具有与未刺激的SK细胞相似的PSI。此外，我们还表明，这种杀伤细胞可以从几个不同的淋巴细胞亚群中诱导产生，并且与SK细胞不同，它们不表达Fc受体。本文讨论了这些发现与SK细胞系统的性质、机制和生物学意义的关系。
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1
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Int J Cancer. 1974 Jun 15;13(6):891-903. doi: 10.1002/ijc.2910130617.
3
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Scand J Immunol. 1974;3(6):739-47. doi: 10.1111/j.1365-3083.1974.tb01309.x.
4
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