Iwasawa Kokoro, Kusumi Akihiro
Membrane Mechanisms Project, ICORP-JST, Institute for Frontier Medical Sciences, Kyoto University.
Nihon Rinsho. 2007 Feb;65(2):219-26.
Recent advancements in single-molecule tracking methods with the nanometer-level precision allow researchers to observe the movement, recruitment, and activation of single molecules in the plasma membrane in living cells. The interaction, binding, and colocalization of two or more molecules in living cells are essential aspects of many biological molecular processes, and single-molecule technologies are very powerful tools for investigating these processes in live cells. Here, we introduce two of these investigation methods, "simultaneous, dual-color, single fluorescent molecule colocalization imaging" and" single molecule FRET(single -molecule fluorescent resonance energy transfer) ", for monitoring the colocalization or binding of two single molecules. Benchmarks have recently been established for these technologies by the research conducted in our laboratory, and thus can now be applied to wide ranges of studies for molecular interactions.
单分子追踪方法在纳米级精度上的最新进展，使研究人员能够观察活细胞中质膜内单个分子的运动、募集和激活。活细胞中两个或更多分子的相互作用、结合和共定位是许多生物分子过程的重要方面，而单分子技术是研究活细胞中这些过程的非常强大的工具。在此，我们介绍其中两种研究方法，即“同步、双色、单荧光分子共定位成像”和“单分子荧光共振能量转移”，用于监测两个单分子的共定位或结合。最近，我们实验室开展的研究已为这些技术建立了基准，因此现在可应用于广泛的分子相互作用研究。
