Zhang Yan, Xie Mei-Lin, Zhu Lu-Jia, Gu Zhen-Lun
Department of Pharmacology, Medical School of Soochow University, Suzhou 215123, China.
Acta Pharmacol Sin. 2007 Mar;28(3):398-403. doi: 10.1111/j.1745-7254.2007.00533.x.
To study the effects of osthole on hyperlipidemic fatty liver and investigate the possible mechanisms.
A rat model with hyperlipidemic fatty liver was successfully established by feeding fatty milk for 6 weeks. The experimental rats were then treated with 5-20 mg/kg osthole for 6 weeks. The mouse hyperlipidemic model was induced by feeding fatty milk when they were treated with 10-20 mg/kg osthole for 3 weeks.
After treatment with osthole, the levels of rat serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol significantly decreased as compared with the fatty liver model group (P< 0.05 or P< 0.01). Hepatic weight and its coefficient, the hepatic tissue contents of TC, TG, and malondialdehyde, also significantly decreased (P< 0.05 or P< 0.01). In fatty milk-induced hyperlipidemic mice, the post-heparin plasma activities of lipoprotein lipase (LPL), hepatic lipase (HL), and total lipase (TL) significantly increased after treatment with 10-20 mg/kg osthole for 3 weeks (P< 0.05 or P< 0.01). Importantly, the histological evaluation of rat liver demonstrated that osthole dramatically decreased lipid accumulation (P< 0.01).
Osthole was found to have therapeutic effects on fatty milk-induced rat fatty liver; the mechanisms might be associated with its anti-oxidation and the elevation of the activities of LPL and HL.
研究蛇床子素对高脂血症性脂肪肝的影响并探讨其可能机制。
通过给予高脂乳剂6周成功建立高脂血症性脂肪肝大鼠模型。然后将实验大鼠用5 - 20 mg/kg蛇床子素治疗6周。当小鼠用10 - 20 mg/kg蛇床子素治疗3周时，通过给予高脂乳剂诱导小鼠高脂血症模型。
用蛇床子素治疗后，与脂肪肝模型组相比，大鼠血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇水平显著降低（P < 0.05或P < 0.01）。肝脏重量及其系数、肝脏组织中TC、TG和丙二醛含量也显著降低（P < 0.05或P < 0.01）。在高脂乳剂诱导的高脂血症小鼠中，用10 - 20 mg/kg蛇床子素治疗3周后，肝素后血浆脂蛋白脂肪酶（LPL）、肝脂肪酶（HL）和总脂肪酶（TL）活性显著升高（P < 0.05或P < 0.01）。重要的是，大鼠肝脏的组织学评估表明蛇床子素显著降低了脂质蓄积（P < 0.01）。
发现蛇床子素对高脂乳剂诱导的大鼠脂肪肝有治疗作用；其机制可能与其抗氧化作用以及LPL和HL活性升高有关。
