Jung Eun-Jung, Moon Hyeong-Gon, Cho Bok Im, Jeong Chi-Young, Joo Young-Tae, Lee Young-Joon, Hong Soon-Chan, Choi Sang-Kyung, Ha Woo-Song, Kim Jae Won, Lee Chang-Won, Lee Jong Sil, Park Soon-Tae
Department of Surgery, College of Medicine, Gyeongsang National University, Gyeongsang National University Hospital, Jinju, Korea.
Int J Cancer. 2007 Jun 1;120(11):2331-8. doi: 10.1002/ijc.22434.
Understanding the molecular background of breast cancer biology is critical in developing new biomarkers for earlier diagnosis and more optimized treatment. We performed a proteomic analysis of human breast carcinoma tissues to investigate the tumor-specific protein expression in breast carcinoma. Using 2-dimensional electorphoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), we were able to identify a list of proteins which are upregulated in cancerous tissue. There was significant increase of galectin-1 expression in all cancerous tissues compared to noncancerous tissues, and its increased expression was further confirmed by western blot immunostaining. Subsequent immunohistochemical staining against galectin-1 in 105 breast cancer specimens showed significant correlation between galectin-1 expression in cancer-associated stromal cells and tumor invasiveness, T stage, TNM stage, and axillary lymph node metastasis. Galectin-1 expressionin cancer cells showed no correlation to above-mentioned pathologic variables. Hormonal receptor status and galectin-1 expression showed no correlation. This study demonstrates the upregulation of galectin-1 in breast carcinoma tissues and the clinical significance of galectin-1 in breast cancer patients. Our data supports the recently highlighted roles of galectin-1 in cancer-associated stroma and in tumor immune privilege.
了解乳腺癌生物学的分子背景对于开发用于早期诊断和更优化治疗的新生物标志物至关重要。我们对人乳腺癌组织进行了蛋白质组学分析，以研究乳腺癌中肿瘤特异性蛋白表达。使用二维电泳（2-DE）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS），我们能够鉴定出一系列在癌组织中上调的蛋白质。与非癌组织相比，所有癌组织中半乳凝素-1的表达均显著增加，并且通过蛋白质印迹免疫染色进一步证实了其表达增加。随后，对105例乳腺癌标本进行的半乳凝素-1免疫组织化学染色显示，癌相关基质细胞中半乳凝素-1的表达与肿瘤侵袭性、T分期、TNM分期和腋窝淋巴结转移之间存在显著相关性。癌细胞中半乳凝素-1的表达与上述病理变量无关。激素受体状态与半乳凝素-1的表达无关。本研究证明了乳腺癌组织中半乳凝素-1的上调以及半乳凝素-1在乳腺癌患者中的临床意义。我们的数据支持了最近强调的半乳凝素-1在癌相关基质和肿瘤免疫豁免中的作用。
