Sawa Shinichiro, Kinoshita Atsuko, Nakanomyo Ikuko, Fukuda Hiroo
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Chem Rec. 2006;6(6):303-10. doi: 10.1002/tcr.20091.
For many years, the plant hormones auxin, cytokinin, ethylene, gibberellin, abscisic acid, brassinosteroid, jasmonic acid, and salicylic acid have been extensively studied as key regulators of plant growth and development. However, recent biochemical and genetic analyses have revealed that secretory peptides are also responsible for intercellular signaling in plants and regulate various events including wound response, cell division control, and pollen self-incompatibility. We discovered two natural CLAVATA3 (CLV3)/ESR-related (CLE) peptides: tracheary elements differentiation inhibitory factor (TDIF) and CLV3, which are dodecapeptides with two hydroxyproline residues that regulate vascular development and meristem formation, respectively. This discovery enabled us to predict the chemical form of CLE gene products. In the Arabidopsis genome, there are 31 CLE genes that correspond to 26 CLE peptides. The application of all 26 chemically synthesized peptides to plants revealed the existence of distinctive functional groups. From these results, we discuss the functions of CLE peptides in plant development and plant-parasite interactions.
多年来，植物激素生长素、细胞分裂素、乙烯、赤霉素、脱落酸、油菜素内酯、茉莉酸和水杨酸作为植物生长发育的关键调节因子受到了广泛研究。然而，最近的生化和遗传学分析表明，分泌肽在植物细胞间信号传导中也发挥作用，并调控包括伤口反应、细胞分裂控制和花粉自交不亲和等各种过程。我们发现了两种天然的CLAVATA3（CLV3）/ESR相关（CLE）肽：管状分子分化抑制因子（TDIF）和CLV3，它们都是具有两个羟脯氨酸残基的十二肽，分别调控维管发育和分生组织形成。这一发现使我们能够预测CLE基因产物的化学形式。在拟南芥基因组中，有31个CLE基因，对应26种CLE肽。将所有26种化学合成肽应用于植物，揭示了不同功能基团的存在。基于这些结果，我们讨论了CLE肽在植物发育和植物-寄生虫相互作用中的功能。
