白细胞介素-24、调节激活正常T细胞表达和分泌因子（RANTES）以及趋化因子受体5（CCR5）基因多态性与成人慢性牙周炎无关。

Interleukin-24, RANTES and CCR5 gene polymorphisms are not associated with chronic adult periodontitis.

作者信息

Savarrio L, Donati M, Carr C, Kinane D F, Berglundh T

机构信息

University of Glasgow, Glasgow, UK.

出版信息

J Periodontal Res. 2007 Apr;42(2):152-8. doi: 10.1111/j.1600-0765.2006.00928.x.

DOI:10.1111/j.1600-0765.2006.00928.x

PMID:17305874

Abstract

BACKGROUND AND OBJECTIVE

Cytokines, such as interleukin-10, and related genetic polymorphisms, have been implicated in the pathogenesis of chronic periodontitis. The aim of this study was to investigate a possible correlation between chronic periodontitis and genetic polymorphisms coding for two interleukin-10 related chemokines [interleukin-24 and regulated on activation, normal T cells expressed and secreted (RANTES)] as well as a RANTES receptor [CC chemokine receptor 5 (CCR5)].

MATERIAL AND METHODS

A single-blind, two-centre, case-controlled study was carried out with test patients from the Clinic of Periodontics, Göteborg University, and from the Department of Periodontology, Glasgow University, and control subjects from the undergraduate clinics of both schools. Blood samples were collected from 106 patients (56 women and 50 men, mean age 51.7 yr) with generalized, severe chronic periodontitis and from 69 periodontally healthy subjects (37 women and 32 men, mean age 53.3 yr). The polymerase chain reaction (PCR) was used to identify the genetic coding for interleukin-24, RANTES and CCR5. Genotype and allele frequencies were compared between the test and control groups using Fischer's exact test at the 5% level of significance.

RESULTS

There were no statistically significant differences between patients with chronic periodontitis and control subjects, regarding genotype distribution or allele frequency, irrespective of smoking status, in the combined Glasgow and Gothenburg cohort or in the specific location cohorts. The allele frequencies for healthy and control subjects for RANTES gave a p-value of 0.80 (allele G was 58.8% in healthy subjects and and 54.4% in subjects with periodontitis), for interleukin-24 the p-value was 0.90 (allele T was 56.2% in healthy subjects and and 54.9% in subjects with periodontitis) and for CCR5 the p-value was 0.90 (the wild-type allele was 85% in healthy subjects and and 82.7% in subjects with periodontitis).

CONCLUSION

The interleukin-24, RANTES and CCR5 polymorphisms investigated are not associated with chronic periodontitis.

摘要

背景与目的

细胞因子，如白细胞介素 - 10，以及相关基因多态性，与慢性牙周炎的发病机制有关。本研究的目的是调查慢性牙周炎与编码两种白细胞介素 - 10相关趋化因子[白细胞介素 - 24和正常T细胞激活后表达和分泌的调节因子（RANTES）]以及一种RANTES受体[CC趋化因子受体5（CCR5）]的基因多态性之间可能存在的相关性。

材料与方法

进行了一项单盲、双中心、病例对照研究，测试患者来自哥德堡大学牙周病诊所和格拉斯哥大学牙周病科，对照受试者来自两校的本科诊所。从106例患有广泛性重度慢性牙周炎的患者（56名女性和50名男性，平均年龄51.7岁）和69名牙周健康受试者（37名女性和32名男性，平均年龄53.3岁）采集血样。采用聚合酶链反应（PCR）鉴定白细胞介素 - 24、RANTES和CCR5的基因编码。使用费舍尔精确检验在5%的显著性水平上比较测试组和对照组之间的基因型和等位基因频率。

结果

在格拉斯哥和哥德堡联合队列或特定地点队列中，无论吸烟状况如何，慢性牙周炎患者与对照受试者在基因型分布或等位基因频率方面均无统计学显著差异。RANTES在健康受试者和对照受试者中的等位基因频率p值为0.80（健康受试者中G等位基因为58.8%，牙周炎患者中为54.4%），白细胞介素 - 24的p值为0.90（健康受试者中T等位基因为56.2%，牙周炎患者中为54.9%），CCR5的p值为0.90（野生型等位基因在健康受试者中为85%，牙周炎患者中为82.7%）。