Fehri S, Tazi I, Loukhmass L, Benchemsi N
Centre régional de transfusion sanguine de Casablanca, rue Faidouzi, BP 5338 Maarif Casablanca, Maroc.
Transfus Clin Biol. 2006 Dec;13(6):335-40. doi: 10.1016/j.tracli.2006.12.001. Epub 2007 Feb 15.
Bacterial contamination of unstable blood products constitutes today the most frequent infectious risk transmitted by blood transfusion. Platelet concentrates are often incrimineted. As responsible germs are in general of cutaneous origin, a sample procedure with diversion of the first 20 ml during blood donation is studied. The aim of this study is to evaluate the results of this technique on bacterial contamination rate of standard platelet units prepared at the regional blood transfusion center in Casablanca.
A comparative study of two types of sample pockets is made: 500 Standard Platelet concentrates (CPS) are prepared after collection using standard triple bags (Baxter) (group 1) and 560 pockets of CPS were prepared after collection using triple bags with Sample Diversion Pouch sampling system for elimination of the first 20 ml of donation (Macopharma and Terumo) (group 2). The skin was disinfected in two times with alcohol 70%. The bacteriological study was made in the two groups at the third day of conservation.
Six CPS of group 1 were contaminated, of which five were staphylococci coagulase negative and one bacillus sp. Six CPS of group 2 were contaminated, of which five were staphylococci coagulase negative and one staphylococcus aureus. The bacteria isolated were those of cutaneous flora at 100%. Diversion of first 20 ml of blood donation results in a 16.6% reduction in bacterial contamination of CPS (P>0.05).
The non-significant reduction in the prevalence of the bacterial infection of CP formulates the problem of the indication of the sampling devices with derivation of first 20 ml during blood collection.
不稳定血液制品的细菌污染是当今输血传播的最常见感染风险。浓缩血小板常被牵连。由于致病细菌一般来源于皮肤，因此研究了在献血过程中抽取前20毫升血液的采样程序。本研究的目的是评估该技术对卡萨布兰卡地区输血中心制备的标准血小板单位细菌污染率的影响。
对两种类型的采样袋进行比较研究：使用标准三袋（百特）采集后制备500份标准浓缩血小板（CPS）（第1组），使用带有采样引流袋采样系统以去除前20毫升献血的三袋采集后制备560袋CPS（麦克法玛和泰尔茂）（第2组）。皮肤用70%酒精消毒两次。在保存第三天对两组进行细菌学研究。
第1组有6份CPS被污染，其中5份为凝固酶阴性葡萄球菌，1份为芽孢杆菌属。第2组有6份CPS被污染，其中5份为凝固酶阴性葡萄球菌，1份为金黄色葡萄球菌。分离出的细菌100%为皮肤菌群。献血时抽取前20毫升血液可使CPS的细菌污染减少16.6%（P>0.05）。
CP细菌感染发生率的降低无统计学意义，提出了采血时抽取前20毫升血液的采样装置的适应证问题。
