Contreras-Ramos S M, Alvarez-Bernal D, Dendooven L
Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Av. Instituto Politécnico Nacional 2508, CP 07360 México, DF, Mexico.
Chemosphere. 2007 May;67(10):2072-81. doi: 10.1016/j.chemosphere.2006.10.069. Epub 2007 Feb 20.
Nitrogen mineralization in PAHs contaminated soil in presence of Eisenia fetida amended with biosolid or vermicompost was investigated. Sterilized and unsterilized soil was contaminated with PAHs, added with E. fetida and biosolid or vermicompost and incubated aerobically for 70 days, while dynamics of inorganic N were monitored. Addition of E. fetida to sterilized soil increased concentration of NH(4)(+) 100> mg N kg(-1), while concentrations in unsterilized remained <60 mg N kg(-1) except for soil amended with biosolid plus PAHs where it increased to >80 mg kg(-1). Addition of PAHs had no significant effect on concentration of NH(4)(+) compared to the unamended soil, except in the soil added with biosolid. Addition of E. fetida to sterilized soil increased concentration of NO(2)(-) 15> mg N kg(-1) while concentrations in unsterilized soil remained <7.5 mg N kg(-1) except for soil amended with biosolid where it increased to >20 mg kg(-1). Addition of PAHs had no significant effect on concentration of NO(2)(-) compared to the unamended soil. Addition of biosolid and vermicompost increased concentration of NO(3)(-), while addition of E. fetida decreased concentration of NO(3)(-) in biosolid amended soil. It was found that NH(4)(+) and NO(2)(-) oxidizers were present in the gut of E. fetida, but their activity was not sufficient enough to inhibit a temporarily increase in concentrations of NH(4)(+) and NO(2)(-). Contamination with PAHs induced immobilization of N in biosolid or vermicompost amended soil, as did feeding of E. fetida on biosolid or vermicompost.
研究了在添加了生物固体或蚯蚓堆肥的情况下，赤子爱胜蚓对多环芳烃污染土壤中氮矿化的影响。将灭菌和未灭菌的土壤用多环芳烃污染，添加赤子爱胜蚓和生物固体或蚯蚓堆肥，并在有氧条件下培养70天，同时监测无机氮的动态变化。向灭菌土壤中添加赤子爱胜蚓使铵离子浓度增加了100>毫克氮/千克(-1)，而未灭菌土壤中的浓度除了添加生物固体和多环芳烃的土壤中增加到>80毫克/千克(-1)外，其余均<60毫克氮/千克(-1)。与未添加多环芳烃的土壤相比，添加多环芳烃对铵离子浓度没有显著影响，但在添加生物固体的土壤中除外。向灭菌土壤中添加赤子爱胜蚓使亚硝酸根离子浓度增加了15>毫克氮/千克(-1)，而未灭菌土壤中的浓度除了添加生物固体的土壤中增加到>20毫克/千克(-1)外，其余均<7.5毫克氮/千克(-1)。与未添加多环芳烃的土壤相比，添加多环芳烃对亚硝酸根离子浓度没有显著影响。添加生物固体和蚯蚓堆肥增加了硝酸根离子浓度，而在添加生物固体的土壤中添加赤子爱胜蚓则降低了硝酸根离子浓度。研究发现，赤子爱胜蚓肠道中存在铵离子和亚硝酸根离子氧化菌，但其活性不足以抑制铵离子和亚硝酸根离子浓度的暂时增加。多环芳烃污染导致生物固体或蚯蚓堆肥改良土壤中氮的固定，赤子爱胜蚓以生物固体或蚯蚓堆肥为食也有同样的效果。
