Anraku Yasutaka, Takahashi Yoshiko, Kitano Hiromi, Hakari Mami
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Colloids Surf B Biointerfaces. 2007 May 15;57(1):61-8. doi: 10.1016/j.colsurfb.2007.01.003. Epub 2007 Jan 14.
A dithiolated random copolymer with pendent phenylboronic acid residues (Cys-Poly(3-acrylamidophenylboronic acid-co-2-dimethylaminopropyl methacrylamide), Cys-Poly(APBA-co-DMAPMA)) obtained by photo-iniferter method was accumulated as a polymer brush on a cap-shaped gold particles deposited on a vacuum-evaporated gold film, and the usefulness of the polymer brush as a sensing element for glycoprotein, ovalbumin (OVA), was examined by using UV-vis spectroscopy with a help of surface plasmon resonance. A similar system was constructed with a dithiolated mannose-carrying polymer, dithiolated-poly(2-methacryloyloxyethyl-D-mannopyranoside) (DT-PMEMan), prepared by the atom transfer radical polymerization (ATRP). The brush composed of this polymer was examined as a sensing element for lectin, concanavalin A (Con A). The sensor cells modified with Cys-Poly(APBA-co-DMAPMA) and DT-PMEMan showed a concentration-dependent binding of OVA and Con A, respectively, with a comparable detection limit to those with a monolayer of polymer brush-coated gold particle deposited on a glass substrate. Using this system, it can be expected to open a new perspective to various functional polymer brushes fixed to the cap-shaped gold particle on a solid substrate.
通过光引发转移终止剂法制备的带有苯基硼酸残基的二硫醇化无规共聚物（半胱氨酸-聚（3-丙烯酰胺基苯基硼酸-co-2-二甲基氨基丙基甲基丙烯酰胺），半胱氨酸-聚（APBA-co-DMAPMA））作为聚合物刷堆积在沉积于真空蒸发金膜上的帽形金颗粒上，并借助表面等离子体共振，利用紫外-可见光谱法研究了该聚合物刷作为糖蛋白卵清蛋白（OVA）传感元件的实用性。使用原子转移自由基聚合（ATRP）制备的带有甘露糖的二硫醇化聚合物二硫醇化-聚（2-甲基丙烯酰氧基乙基-D-甘露吡喃糖苷）（DT-PMEMan）构建了类似的体系。研究了由该聚合物组成的刷作为凝集素伴刀豆球蛋白A（Con A）传感元件的性能。用半胱氨酸-聚（APBA-co-DMAPMA）和DT-PMEMan修饰的传感器单元分别显示出OVA和Con A的浓度依赖性结合，其检测限与沉积在玻璃基板上的聚合物刷涂层金颗粒单层的检测限相当。利用该体系，有望为固定在固体基板上帽形金颗粒上的各种功能聚合物刷开辟新的前景。
