Nakazawa Yozo, Uchino Masataka, Sagane Yoshimasa, Sato Hiroaki, Takano Katsumi
Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Microbiol Res. 2009;164(1):43-8. doi: 10.1016/j.micres.2006.11.003. Epub 2007 Feb 20.
The present study was conducted to screen microorganisms that produce phospholipase D (PLD), and we especially focused on the strains having high transphosphatidylation activity. Eighty bacterial strains were isolated from soil samples by a screening method utilizing a preliminary selection medium with phosphatidylcholine (PC) as the sole carbon source. The culture supernatants were then assayed for PLD activity. The finding of dual PLD activities in cultures revealed that the hydrolytic and transphosphatidylation activities were correlated. Consequently, six strains were selected as stably producing PLD enzyme(s) during continuous subcultures. The culture supernatants of selected strains synthesized phosphatidylglycerol, phosphatidylserine and phosphatidylethanolamine from PC with high conversion rates. These isolated strains will be made available to carry out phospholipid modification through the efficient transphosphatidylation activity of the PLD that they produce.
本研究旨在筛选产磷脂酶D（PLD）的微生物，我们特别关注具有高转磷脂酰化活性的菌株。通过利用以磷脂酰胆碱（PC）作为唯一碳源的初步筛选培养基的筛选方法，从土壤样品中分离出80株细菌菌株。然后测定培养上清液的PLD活性。培养物中双重PLD活性的发现表明水解活性和转磷脂酰化活性是相关的。因此，选择了6株在连续传代培养过程中稳定产生PLD酶的菌株。所选菌株的培养上清液以高转化率从PC合成了磷脂酰甘油、磷脂酰丝氨酸和磷脂酰乙醇胺。这些分离出的菌株将可用于通过它们所产生的PLD的高效转磷脂酰化活性进行磷脂修饰。
