Pramoonjago P, Kinoshita T, Hong K S, Takata-Kozono Y, Kozono H, Inagi R, Inoue K
Department of Bacteriology, Osaka University Medical School, Japan.
J Immunol. 1992 Feb 1;148(3):837-43.
Escherichia coli B/SM, strain 1-1, was killed dose dependently by human hereditary C9-deficient serum (C9DHS), which was shown to contain no C9 Ag by an ELISA method. On the other hand, human hereditary C7-deficient serum did not kill the bacteria under similar conditions. The bactericidal activity of C9DHS was inhibited by rabbit anti-C5 antibody but not by murine anti-C9 mAb. The anti-C9 antibody decreased the bactericidal activity of normal human serum (NHS) to the level of that with C9DHS. Sheep anti-human lysozyme antibody did not affect the bactericidal activity of C9DHS or NHS even when added at more than twice the concentration required to block the serum lysozyme activity on Micrococcus luteus. After treatment with C9DHS and washing, surviving Escherichia coli were killed by C9, but not by lysozyme, transferrin, or both. Other strains of E. coli (K12 W3110, C600, and NIHJ) and Salmonella typhimurium (strain NCTC 74), all maintained in the laboratory, were also killed by C9DHS. However, pathogenic strains recently isolated from patients with traveler's diarrhea and some strains of S. typhimurium were resistant to both C9DHS and NHS, at least at the serum concentration tested. A concentration of 0.1 M Tris did not increase the susceptibility of serum-resistant strains of bacteria to C9DHS, but made one strain of S. typhimurium tested susceptible to NHS, but not to C9DHS. These results clearly showed that C9DHS kills bacteria that are sensitive to NHS through activation of C up to the step of C8 in the same way that C9-deficient C serum lyzed sensitized erythrocytes.
大肠杆菌B/SM 1-1菌株被人遗传性C9缺陷血清（C9DHS）以剂量依赖方式杀灭，ELISA法显示该血清不含C9抗原。另一方面，人遗传性C7缺陷血清在类似条件下未杀灭该细菌。C9DHS的杀菌活性被兔抗C5抗体抑制，但不被鼠抗C9单克隆抗体抑制。抗C9抗体将正常人血清（NHS）的杀菌活性降低至C9DHS的水平。羊抗人溶菌酶抗体即使以超过阻断对藤黄微球菌血清溶菌酶活性所需浓度两倍的量添加，也不影响C9DHS或NHS的杀菌活性。用C9DHS处理并洗涤后，存活的大肠杆菌被C9杀灭，但不被溶菌酶、转铁蛋白或两者共同杀灭。实验室保存的其他大肠杆菌菌株（K12 W3110、C600和NIHJ）以及鼠伤寒沙门氏菌（菌株NCTC 74）也被C9DHS杀灭。然而，最近从旅行者腹泻患者中分离出的致病菌株以及一些鼠伤寒沙门氏菌菌株至少在所测试的血清浓度下对C9DHS和NHS均有抗性。0.1 M Tris的浓度并未增加血清抗性细菌菌株对C9DHS的敏感性，但使所测试的一株鼠伤寒沙门氏菌对NHS敏感，但对C9DHS不敏感。这些结果清楚地表明，C9DHS通过将补体激活至C8步骤来杀灭对NHS敏感的细菌，方式与C9缺陷的补体血清裂解致敏红细胞相同。
