Johnson J R, Berggren T, Manivel J C
Department of Medicine, University of Minnesota Medical School, Minneapolis.
J Infect Dis. 1992 Feb;165(2):299-305. doi: 10.1093/infdis/165.2.299.
To clarify the usefulness of histopathology in evaluating invasiveness during acute cystitis and pyelonephritis in a mouse model of urinary tract infection, findings from bladder and kidney sections of mice inoculated transurethrally with Escherichia coli were compared with results of bladder, kidney, spleen, and blood cultures and with changes in peripheral blood leukocyte counts. All of the 14 bladder histopathologic abnormalities evaluated were significantly associated with a positive bladder culture, and 7 were associated with splenic infection. Histopathologic features of cystitis were present in some culture-negative bladders. Eleven of 12 renal histopathologic abnormalities evaluated were significantly associated both with a positive kidney culture and with splenic infection, and two correlated with the development of peripheral leukocytosis. Histopathologic features of pyelitis and nephritis permitted culture-positive kidneys to be categorized as exhibiting colonization only, pyelitis only, or pyelitis plus frank nephritis and demonstrated that some culture-negative kidneys exhibit signs of pyelitis and nephritis. These findings suggest that detailed, semiquantitative histopathologic evaluation can add to quantitative cultures in the assessment of bacterial urovirulence in the mouse model of ascending urinary tract infection.
为了阐明组织病理学在评估尿路感染小鼠模型急性膀胱炎和肾盂肾炎期间侵袭性方面的作用，将经尿道接种大肠杆菌的小鼠膀胱和肾脏切片的结果与膀胱、肾脏、脾脏和血液培养结果以及外周血白细胞计数的变化进行了比较。评估的14项膀胱组织病理学异常均与膀胱培养阳性显著相关，7项与脾脏感染相关。膀胱炎的组织病理学特征在一些培养阴性的膀胱中也存在。评估的12项肾脏组织病理学异常中有11项与肾脏培养阳性和脾脏感染均显著相关，两项与外周白细胞增多的发生相关。肾盂炎和肾炎的组织病理学特征使培养阳性的肾脏可被分类为仅表现为定植、仅表现为肾盂炎或肾盂炎加明显肾炎，并表明一些培养阴性的肾脏表现出肾盂炎和肾炎的迹象。这些发现表明，在评估上行性尿路感染小鼠模型中的细菌尿毒力时，详细的半定量组织病理学评估可以补充定量培养。
