Cao Yueqing, Peng Guoxiong, He Zhengbo, Wang Zhongkang, Yin Youping, Xia Yuxian
Genetic Engineering Research Center, Institute of Bioengineering, Chongqing University, Chongqing, PR China.
Biotechnol Lett. 2007 Jun;29(6):907-11. doi: 10.1007/s10529-007-9332-7. Epub 2007 Feb 20.
A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the haemolymph of the infected locust, and the benomyl-resistance was maintained. Results suggested that the two markers provided a useful tool for screening and monitoring the engineered strains even after infection.
