Kanwar J R, Kaushik S P, Sawhney I M, Kamboj M S, Mehta S K, Vinayak V K
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
J Med Microbiol. 1992 Jan;36(1):46-51. doi: 10.1099/00222615-36-1-46.
Hydatid fluids from sheep, goat, pig and man, after resolution by sodium dodecyl sulphate-polyacrylamide gel electrophoresis under reducing conditions, revealed at least 15 discrete polypeptide bands of 8-116 Kda. By ELISA, sera from all 20 cases of hydatidosis showed anti-hydatid antibody, but so did 11 (73%) of 15 sera samples from cysticercosis patients, eight (67%) of 12 sera from patients with other parasitic infections (amoebic liver abscess or hymenolepiasis) and one (4%) of 25 sera from healthy controls. Antibody to cysticercus antigen was found in 14 (93%) of 15 sera from cysticercosis patients, 17 (85%) of 20 sera from hydatid patients, six (50%) of 12 sera from patients with other parasitic infections and one (4%) of 25 sera from healthy controls. Sera from 17 (85%) of 20 hydatid patients, 11 (73%) of 15 cysticercosis patients and five (42%) of 12 patients with other parasitic infections had antibodies to both hydatid and cysticercus antigens. Sera from 20 surgically confirmed cases of hydatidosis reacted with 12 polypeptides of 8-116 Kda in Western immunoblot with hydatid antigens. Polypeptides of 16, 24, 38, 45 and 58 Kda were recognised by all hydatidosis sera but also by many sera from patients with other infections. However, polypeptides of 8 and 116 Kda were recognised by all hydatidosis sera but not by any sera from patients with cysticercosis, other parasitic infections or viral hepatitis, or from healthy controls. Thus, recognition of 8- and 116-Kda hydatid antigens by a patient's serum appears to be a specific test confirming a clinical diagnosis in an individual case of hydatidosis.
绵羊、山羊、猪和人的棘球蚴液在还原条件下经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离后，显示出至少15条分子量为8 - 116 kDa的离散多肽带。通过酶联免疫吸附测定法（ELISA），20例棘球蚴病患者的血清均显示有抗棘球蚴抗体，但15例囊尾蚴病患者的血清中有11例（73%）、12例其他寄生虫感染（阿米巴肝脓肿或微小膜壳绦虫病）患者的血清中有8例（67%）以及25例健康对照者的血清中有1例（4%）也呈阳性。15例囊尾蚴病患者的血清中有14例（93%）、20例棘球蚴病患者的血清中有17例（85%）、12例其他寄生虫感染患者的血清中有6例（50%）以及25例健康对照者的血清中有1例（4%）检测到抗囊尾蚴抗原抗体。20例棘球蚴病患者中有17例（85%）的血清、15例囊尾蚴病患者中有11例（73%）的血清以及12例其他寄生虫感染患者中有5例（42%）的血清同时含有抗棘球蚴和抗囊尾蚴抗原抗体。20例经手术确诊的棘球蚴病患者的血清在与棘球蚴抗原进行的Western免疫印迹中，与8 - 116 kDa的12种多肽发生反应。16、24、38、45和58 kDa的多肽能被所有棘球蚴病血清识别，但也能被许多其他感染患者的血清识别。然而，8和116 kDa的多肽能被所有棘球蚴病血清识别，但不能被囊尾蚴病患者、其他寄生虫感染患者或病毒性肝炎患者的血清以及健康对照者的血清识别。因此，患者血清对8 kDa和116 kDa棘球蚴抗原的识别似乎是一项确诊个体棘球蚴病临床诊断的特异性检测。
