Gupta Smriti, Ciungu Alexandra, Jameson Natalie, Lal Shailesh K
Department of Biological Sciences, Oakland University, Rochester, MI 48309-4401, USA.
DNA Seq. 2006 Aug;17(4):254-61. doi: 10.1080/10425170600856642.
A U1-snRNP--specific 70K (U1-70K) protein is intricately involved in both constitutive and alternative splicing of pre-mRNAs. Here, we report cDNA and cognate genomic sequences of the U1-70K gene of maize and rice. The maize and rice U1-70K genes bear strong similarity to the Arabidopsis gene and each encode three transcripts in roots and shoots. Alternative splicing produces two transcripts from each gene in addition to the mRNA encoding the wild type protein. In both cases, selective inclusion of intron 6 or utilization of a cryptic donor site within intron 6 sequence generates the two alternatively spliced transcripts. This evolutionary conservation of splicing patterns between different plant species suggests an important biological function for alternative splicing in the expression of U1-70K gene.
一种U1 - snRNP特异性70K（U1 - 70K）蛋白复杂地参与前体mRNA的组成型剪接和可变剪接。在此，我们报道了玉米和水稻U1 - 70K基因的cDNA及相关基因组序列。玉米和水稻的U1 - 70K基因与拟南芥基因具有高度相似性，并且在根和芽中各自编码三种转录本。可变剪接除了产生编码野生型蛋白的mRNA外，还从每个基因产生另外两种转录本。在这两种情况下，内含子6的选择性包含或内含子6序列内一个隐蔽供体位点的利用产生了两种可变剪接的转录本。不同植物物种间这种剪接模式的进化保守性表明可变剪接在U1 - 70K基因表达中具有重要的生物学功能。
