Chen Fei, Ji Chaoneng, Dou Tonghai, Zheng Nan, Qiu Rui, Peng Jing, Fang Weiqun, Feng Congjing, Xie Yi, Mao Yumin
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
DNA Seq. 2006 Aug;17(4):282-6. doi: 10.1080/10425170600807744.
Pre-mRNA splicing allows individual genes to produce multiple protein isoforms with diverse functions. Recognition of functional splice sites in pre-mRNAs is very important in this splicing process and requires some protein auxiliary factors such as U2 small nuclear ribonucleoprotein auxiliary factor small subunit (U2AF35, encoded by U2AF1). By its RNA binding domains, U2AF35 interacts with U2AF65 to bind 3' splice site of pre-mRNA and initiates splicing. Another protein, which is named as U2AF1-like3 (U2AF1L3), shows high similarity with U2AF35 and may have related function in pre-mRNA splicing. Here, we report a splice variant of U2AF1L3, which is 767 bp in length and has an open reading frame (ORF) coding a predicted 181 amino acids protein. Reverse transcription-PCR (RT-PCR) shows that this isoform has different expression pattern with U2AF1L3 and is highly expressed in heart, brain and lung.
前体mRNA剪接使单个基因能够产生具有多种功能的多种蛋白质异构体。在前体mRNA中识别功能性剪接位点在这个剪接过程中非常重要，并且需要一些蛋白质辅助因子，如U2小核核糖核蛋白辅助因子小亚基（U2AF35，由U2AF1编码）。通过其RNA结合结构域，U2AF35与U2AF65相互作用以结合前体mRNA的3'剪接位点并启动剪接。另一种蛋白质，名为U2AF1样3（U2AF1L3），与U2AF35具有高度相似性，并且可能在前体mRNA剪接中具有相关功能。在这里，我们报道了一种U2AF1L3的剪接变体，其长度为767 bp，具有一个开放阅读框（ORF），编码一个预测的181个氨基酸的蛋白质。逆转录PCR（RT-PCR）表明，这种异构体与U2AF1L3具有不同的表达模式，并且在心脏、大脑和肺中高表达。
