• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

溴化脂质可识别球形红杆菌反应中心表面的脂质结合位点。

Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides.

作者信息

Roszak Aleksander W, Gardiner Alastair T, Isaacs Neil W, Cogdell Richard J

机构信息

Department of Chemistry, WestCHEM.

出版信息

Biochemistry. 2007 Mar 20;46(11):2909-16. doi: 10.1021/bi062154i. Epub 2007 Feb 23.

DOI:10.1021/bi062154i
PMID:17315985
Abstract

This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

摘要

本研究描述了使用溴化磷脂来区分典型α-螺旋膜蛋白表面的脂质结合位点和去污剂结合位点。从球形红细菌中分离出的反应中心与添加的溴化磷脂共结晶。对这些晶体的X射线结构分析揭示了由于溴原子具有特征性的强X射线散射而存在两个脂质结合位点。这些结果证明了这种方法在绘制膜蛋白表面脂质结合位点方面的实用性。

相似文献

1
Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides.溴化脂质可识别球形红杆菌反应中心表面的脂质结合位点。
Biochemistry. 2007 Mar 20;46(11):2909-16. doi: 10.1021/bi062154i. Epub 2007 Feb 23.
2
New crystal form of the photosynthetic reaction centre from Rhodobacter sphaeroides of improved diffraction quality.球形红杆菌光合反应中心的新型晶体形式，其衍射质量得到改善。
J Mol Biol. 1993 Apr 20;230(4):1311-4. doi: 10.1006/jmbi.1993.1246.
3
Lipidic sponge phase crystallization of membrane proteins.膜蛋白的脂质海绵相结晶
J Mol Biol. 2006 Nov 17;364(1):44-53. doi: 10.1016/j.jmb.2006.06.043. Epub 2006 Jul 7.
4
Protein regulation of carotenoid binding; gatekeeper and locking amino acid residues in reaction centers of Rhodobacter sphaeroides.类胡萝卜素结合的蛋白质调控；球形红杆菌反应中心中的守门和锁定氨基酸残基
Structure. 2004 May;12(5):765-73. doi: 10.1016/j.str.2004.02.037.
5
Correlating ultrafast function with structure in single crystals of the photosynthetic reaction center.光合反应中心单晶中超快功能与结构的关联。
Biochemistry. 2008 Nov 4;47(44):11387-9. doi: 10.1021/bi801026g. Epub 2008 Oct 11.
6
Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: monitoring the optical properties of the complex from bacterial cell to crystal.来自球形红细菌天线缺陷菌株的野生型和突变型反应中心的结构研究：监测从细菌细胞到晶体的复合物的光学性质。
Biochemistry. 1998 Apr 7;37(14):4740-50. doi: 10.1021/bi971717a.
7
Mapping lipid and detergent molecules at the surface of membrane proteins.绘制膜蛋白表面的脂质和去污剂分子图谱。
Biochem Soc Trans. 2011 Jun;39(3):775-9. doi: 10.1042/BST0390775.
8
pH modulates the quinone position in the photosynthetic reaction center from Rhodobacter sphaeroides in the neutral and charge separated states.pH调节球形红细菌光合反应中心在中性和电荷分离状态下醌的位置。
J Mol Biol. 2007 Aug 10;371(2):396-409. doi: 10.1016/j.jmb.2007.04.082. Epub 2007 May 10.
9
Secrets of carotenoid binding.类胡萝卜素结合的奥秘。
Structure. 2004 May;12(5):733-4. doi: 10.1016/j.str.2004.04.006.
10
[Spatial structure of light-harvesting chlorophyll-protein complex B800-850 from the photosynthesizing bacterium Rhodobacter sphaeroides].[来自光合细菌球形红杆菌的捕光叶绿素蛋白复合物B800 - 850的空间结构]
Mol Biol (Mosk). 1996 Sep-Oct;30(5):1209-18.

引用本文的文献

1
Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.用于阐明膜蛋白结构-功能关系的重原子去污剂/脂质联合X射线晶体学
Membranes (Basel). 2021 Oct 27;11(11):823. doi: 10.3390/membranes11110823.
2
Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent.在使用碘代去污剂的串联飞秒晶体学中，通过单波长反常散射法（SAD）、直接法（SIR）或单波长反常散射直接法（SIRAS）相位测定膜蛋白结构。
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13039-13044. doi: 10.1073/pnas.1602531113. Epub 2016 Oct 31.
3
Extension of the GLYCAM06 Biomolecular Force Field to Lipids, Lipid Bilayers and Glycolipids.
GLYCAM06生物分子力场向脂质、脂质双层和糖脂的扩展。
Mol Simul. 2008;34(4):349-363. doi: 10.1080/08927020701710890.
4
Gating and regulation of the cytochrome c oxidase proton pump.细胞色素c氧化酶质子泵的门控与调节
Biochim Biophys Acta. 2012 Apr;1817(4):489-94. doi: 10.1016/j.bbabio.2011.11.018. Epub 2011 Dec 8.
5
Cardiolipin-based respiratory complex activation in bacteria.基于心磷脂的细菌呼吸复合物激活。
Proc Natl Acad Sci U S A. 2011 May 10;108(19):7781-6. doi: 10.1073/pnas.1010427108. Epub 2011 Apr 25.
6
Overcoming barriers to membrane protein structure determination.克服膜蛋白结构测定的障碍。
Nat Biotechnol. 2011 Apr;29(4):335-40. doi: 10.1038/nbt.1833.
7
Effects of impurities on membrane-protein crystallization in different systems.杂质对不同体系中膜蛋白结晶的影响。
Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1062-73. doi: 10.1107/S0907444909029163. Epub 2009 Sep 16.
8
Conserved lipid-binding sites in membrane proteins: a focus on cytochrome c oxidase.膜蛋白中保守的脂质结合位点：以细胞色素c氧化酶为重点
Curr Opin Struct Biol. 2007 Aug;17(4):444-50. doi: 10.1016/j.sbi.2007.07.001. Epub 2007 Aug 23.