Hall Andrea E, Patel Pratiksha R, Domanski Paul J, Prater Bradley D, Gorovits Elena L, Syribeys Peter J, Vernachio John H, Patti Joseph M, Hutchins Jeff T
Inhibitex, Inc., Alpharetta, Georgia 30004, USA.
Hybridoma (Larchmt). 2007 Feb;26(1):28-34. doi: 10.1089/hyb.2006.039.
Staphylococcus epidermidis is an important opportunistic human pathogen that has recently emerged as a major cause of foreign-body infections. The most important stage contributing to the pathogenesis of this bacteria is the initial adherence to host tissue. SdrG is a cell-wall-anchored fibrinogen-binding adhesin of S. epidermidis that has been shown to be necessary for bacterial binding to fibrinogen-coated foreign bodies, such as catheters. Here we report the generation and characterization of a panel of monoclonal antibodies (MAbs) directed against this S. epidermidis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance, and flow cytometry, we have characterized a diverse array of MAbs that may prove to be beneficial in studies that address the precise biologic role of SdrG.
表皮葡萄球菌是一种重要的人类机会致病菌，最近已成为异物感染的主要原因。导致这种细菌发病机制的最重要阶段是最初对宿主组织的黏附。SdrG是表皮葡萄球菌的一种细胞壁锚定纤维蛋白原结合黏附素，已证明它对于细菌与纤维蛋白原包被的异物（如导管）的结合是必需的。在此，我们报告了一组针对这种表皮葡萄球菌毒力因子的单克隆抗体（MAb）的产生和特性。通过使用多种体外试验、表面等离子体共振和流式细胞术，我们已对一系列不同的单克隆抗体进行了特性分析，这些单克隆抗体可能在研究SdrG的确切生物学作用中被证明是有益的。
