Mahdiyoun S, Deshmukh G D, Abe A, Radin N S, Shayman J A
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0676.
Arch Biochem Biophys. 1992 Feb 1;292(2):506-11. doi: 10.1016/0003-9861(92)90023-p.
Recent work has demonstrated the enhancement of hormone-stimulated inositol trisphosphate formation in renal epithelial cells under conditions of glucosylceramide depletion. The role of glucosylceramide metabolism was explored further by exposing Madin-Darby canine kidney (MDCK) cells to the beta-glucosidase inhibitor conduritol B epoxide, which produced time-dependent and concentration-dependent increases in glucosylceramide levels and decreased bradykinin-stimulated inositol trisphosphate formation from isolated MDCK cell membranes. These data provide further support for an association between glucosylceramide levels and hormone-stimulated inositol trisphosphate formation.
最近的研究表明，在葡萄糖神经酰胺耗竭的情况下，肾上皮细胞中激素刺激的肌醇三磷酸形成会增强。通过将Madin-Darby犬肾（MDCK）细胞暴露于β-葡萄糖苷酶抑制剂环氧扁枝衣霉素，进一步探讨了葡萄糖神经酰胺代谢的作用，该抑制剂使葡萄糖神经酰胺水平呈时间和浓度依赖性增加，并降低了从分离的MDCK细胞膜中缓激肽刺激的肌醇三磷酸形成。这些数据为葡萄糖神经酰胺水平与激素刺激的肌醇三磷酸形成之间的关联提供了进一步的支持。
