Lahiri B, Lai P S, Pousada M, Stanton D, Danishefsky I
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Arch Biochem Biophys. 1992 Feb 14;293(1):54-60. doi: 10.1016/0003-9861(92)90364-3.
Treatment of porcine heparin with the ferrous-EDTA complex and ascorbic acid for 24 h at 37 degrees C results in the degradation of most of the glycosaminoglycan to smaller fragments. About 65% of the products comprise oligosaccharides composed of less than 30 sugar units. The extent of depolymerization is decreased significantly if ascorbate or EDTA is not included in the reaction mixture. Gel filtration of the reaction products yielded fractions with narrow chain length ranges. The sulfate content of the fractions and their electrophoretic mobilities on cellulose acetate indicate that the components have equivalent charge densities. Depolymerization products with 20 or more sugar units retain significant anticoagulant potencies as measured by their effect in accelerating the neutralization of factor Xa by antithrombin.
将猪肝素与亚铁 - 乙二胺四乙酸络合物和抗坏血酸在37℃下处理24小时，会导致大部分糖胺聚糖降解为较小的片段。约65%的产物为糖单元少于30个的寡糖。如果反应混合物中不包含抗坏血酸盐或乙二胺四乙酸，解聚程度会显著降低。对反应产物进行凝胶过滤得到链长范围较窄的馏分。这些馏分的硫酸盐含量及其在醋酸纤维素上的电泳迁移率表明，各组分具有相同的电荷密度。通过其加速抗凝血酶对因子Xa中和作用的效果测定，含有20个或更多糖单元的解聚产物保留了显著的抗凝效力。
