Nicaise Valérie, Gallois Jean-Luc, Chafiai Faccila, Allen Leah M, Schurdi-Levraud Valérie, Browning Karen S, Candresse Thierry, Caranta Carole, Le Gall Olivier, German-Retana Sylvie
Interactions Plante-Virus (IPV), UMR 1090, INRA Bordeaux 2, BP 81, F-33883 Villenave d'Ornon, France.
FEBS Lett. 2007 Mar 6;581(5):1041-6. doi: 10.1016/j.febslet.2007.02.007. Epub 2007 Feb 14.
The translation initiation factors eIF4E and eIF(iso)4E play a key role during virus infection in plants. During mRNA translation, eIF4E provides the cap-binding function and is associated with the protein eIF4G to form the eIF4F complex. Susceptibility analyses of Arabidopsis mutants knocked-out for At-eIF4G genes showed that eIF4G factors are indispensable for potyvirus infection. The colonization pattern by a viral recombinant carrying GFP indicated that eIF4G is involved at a very early infection step. Like eIF4E, eIF4G isoforms are selectively recruited for infection. Moreover, the eIF4G selective involvement parallels eIF4E recruitment. This is the first report of a coordinated and selective recruitment of eIF4E and eIF4G factors, suggesting the whole eIF4F recruitment.
翻译起始因子eIF4E和eIF(iso)4E在植物病毒感染过程中起关键作用。在mRNA翻译过程中，eIF4E提供帽结合功能，并与蛋白质eIF4G结合形成eIF4F复合物。对敲除At-eIF4G基因的拟南芥突变体进行的敏感性分析表明，eIF4G因子对于马铃薯Y病毒感染是不可或缺的。携带绿色荧光蛋白（GFP）的病毒重组体的定殖模式表明，eIF4G参与了病毒感染的非常早期阶段。与eIF4E一样，eIF4G异构体被选择性地募集用于感染。此外，eIF4G的选择性参与与eIF4E的募集平行。这是关于eIF4E和eIF4G因子协同和选择性募集的首次报道，表明整个eIF4F被募集。
