肿瘤坏死因子α在脑血管内皮细胞中的运输
TNFalpha trafficking in cerebral vascular endothelial cells.
作者信息
Pan Weihong, Kastin Abba J, Daniel Jeremy, Yu Chuanhui, Baryshnikova Larisa M, von Bartheld Christopher S
机构信息
Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
出版信息
J Neuroimmunol. 2007 Apr;185(1-2):47-56. doi: 10.1016/j.jneuroim.2007.01.005. Epub 2007 Feb 20.
Abstract
Using small tags, we tracked the pathway of tumor necrosis factor (TNF)alpha across cerebral vascular endothelial cells. In cerebral microvessel derived RBE4 cells, (125)I-TNFalpha had rapid endocytosis within the first 20 min and showed substantial exocytosis in the intact form. Biotinylated TNFalpha was detected at different time points after endocytosis by streptavidin-Quantum dots which showed its time-dependent colocalization with intracellular organelles. In mice, electron microscopic autoradiography after intravenous injection of (125)I-TNFalpha showed its transcytosis, as signals emerged on the abluminal side of the endothelial cells and reached brain parenchyma. The vesicular trafficking of TNFalpha reflects the immunomodulatory potential of peripheral cytokines for the CNS.
摘要
我们使用小标签追踪肿瘤坏死因子（TNF）α穿过脑血管内皮细胞的途径。在源自脑微血管的RBE4细胞中，（125）I-TNFα在最初20分钟内具有快速内吞作用，并以完整形式显示出大量胞吐作用。通过链霉亲和素-量子点在内吞作用后的不同时间点检测到生物素化的TNFα，其显示出与细胞内细胞器的时间依赖性共定位。在小鼠中，静脉注射（125）I-TNFα后的电子显微镜放射自显影显示其转胞吞作用，因为信号出现在内皮细胞的无腔侧并到达脑实质。TNFα的囊泡运输反映了外周细胞因子对中枢神经系统的免疫调节潜力。
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