Levy Einat, Fleisher-Berkovich Sigal
Department of Clinical Pharmacology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel.
Peptides. 2007 Apr;28(4):845-50. doi: 10.1016/j.peptides.2007.01.013. Epub 2007 Jan 30.
The aim of the present study was to investigate the short-term effect of bradykinin on the two cyclooxygenase species in neonatal rat glial cells. In spite of the fact that cyclooxygenase protein levels were not altered, an increase in cyclooxygenase activity was observed. Use of cyclooxygenase-1 inhibitors and paracetamol resulted in complete elimination of the bradykinin-induced prostaglandin E(2) synthesis and of cyclooxygenase enzyme activity. Cyclooxygenase-2 inhibitors only partially inhibited enzyme activity and prostaglandin production. Our data suggest that cyclooxygenase-1 is probably the major contributor to short-term modulation of glial prostaglandin E2 synthesis by bradykinin.
本研究的目的是调查缓激肽对新生大鼠神经胶质细胞中两种环氧化酶的短期作用。尽管环氧化酶蛋白水平未发生改变，但观察到环氧化酶活性增加。使用环氧化酶-1抑制剂和对乙酰氨基酚可完全消除缓激肽诱导的前列腺素E2合成及环氧化酶活性。环氧化酶-2抑制剂仅部分抑制酶活性和前列腺素生成。我们的数据表明，环氧化酶-1可能是缓激肽对神经胶质细胞前列腺素E2合成进行短期调节的主要因素。
