Tuttle W C, Jones R K
Am J Clin Pathol. 1975 Oct;64(4):477-82. doi: 10.1093/ajcp/64.4.477.
The histologic distribution of alpha-1-antitrypsin in frozen sections prepared from four specimens of human lung was determined by the indirect fluorescent antibody technic. Three of the specimens were obtained directly from surgical procedures and were peripheral tissue excised with tumors. The tumors were a mixed-cell "scar cancer" (Case 1), a bronchiolar carcinoma (Case 2), and a benign hemartoma (Case 3). The fourth specimen (Case 4) was obtained at autopsy following death by myocardial infarction. Specific fluorescence for alpha-1-antitrypsin was observed lining the terminal airways and alveoli throughout the sections from Cases 1 and 4. In specimens from Cases 2 and 3, a few focal areas of specific fluorescence were observed. The results of this study suggest that alpha-1-antitrypsin may be distributed in lung in variable concentration in association with pulmonary surfactant.
采用间接荧光抗体技术测定了取自4例人肺标本的冰冻切片中α-1-抗胰蛋白酶的组织学分布。其中3例标本直接取自外科手术，为切除肿瘤时一并切除的外周组织。肿瘤分别为混合细胞型“瘢痕癌”（病例1）、细支气管癌（病例2）和良性错构瘤（病例3）。第4例标本（病例4）是在心肌梗死死亡患者尸检时获得的。在病例1和病例4的切片中，可见终末气道和肺泡内衬有α-1-抗胰蛋白酶的特异性荧光。在病例2和病例3的标本中，仅观察到少数几个特异性荧光灶。本研究结果提示，α-1-抗胰蛋白酶可能以不同浓度与肺表面活性物质相关联地分布于肺内。
