肌动蛋白分子在体外促进鸡端脑神经元的轴突生长。

Actin molecules promote neurite outgrowth of chick telencephalic neurons in vitro.

作者信息

Nobusada H, Taguchi T

机构信息

Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Jan 15;182(1):39-44. doi: 10.1016/s0006-291x(05)80109-3.

DOI:10.1016/s0006-291x(05)80109-3

PMID:1731797

Abstract

Chick brain extract contains protein factors for neurite outgrowth from embryonic chick telencephalic neurons in dissociated cell cultures. In the course of purification of the factors using a bioassay system, a 41 kDa protein was detected to be one of the factors. Immunoblot analysis showed that this protein is identical to actin. In addition, G-actin purified from rabbit skeletal muscle exhibits neurite outgrowth activity in the same dose-dependent manner as the 41 kDa protein. The same bioassay carried with other purified proteins did not show such a remarkable activity.

摘要

鸡脑提取物含有促进解离细胞培养中胚胎鸡端脑神经元神经突生长的蛋白质因子。在用生物测定系统纯化这些因子的过程中，检测到一种41 kDa的蛋白质是其中一个因子。免疫印迹分析表明，这种蛋白质与肌动蛋白相同。此外，从兔骨骼肌中纯化的G-肌动蛋白以与41 kDa蛋白质相同的剂量依赖性方式表现出神经突生长活性。对其他纯化蛋白质进行的相同生物测定未显示出如此显著的活性。