Guntaka R V, Kandala J C, Reddy V D
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.
Biochem Biophys Res Commun. 1992 Jan 15;182(1):412-9. doi: 10.1016/s0006-291x(05)80160-3.
A novel gene encoding a protein of 147 amino acids (Pf16) has been cloned from Plasmodium falciparum and expressed in E. coli. The protein contains 19 methionines, all of which are localized in the NH2-terminal 35 amino acid residues, and it is also rich in lysine. Pf16 is highly basic, contains a polyacidic domain consisting of aspartic acid and is related to the non-histone high mobility group proteins of higher eukaryotes. The gene is conserved among eight different species of Plasmodium so far examined, suggesting an important function for this gene product in the parasite's life cycle.
从恶性疟原虫中克隆出了一个编码147个氨基酸的新型基因（Pf16），并在大肠杆菌中进行了表达。该蛋白质含有19个甲硫氨酸，所有这些甲硫氨酸都位于氨基末端的35个氨基酸残基中，并且富含赖氨酸。Pf16具有高度碱性，包含一个由天冬氨酸组成的多酸性结构域，并且与高等真核生物的非组蛋白高迁移率族蛋白相关。到目前为止，该基因在已检测的八种不同疟原虫物种中是保守的，这表明该基因产物在寄生虫生命周期中具有重要功能。
