Mezzetti A, Lapenna D, Calafiore A M, Proietti-Franceschilli G, Porreca E, De Cesare D, Neri M, Di Ilio C, Cuccurullo F
Laboratorio di Fisiopatologia Medica Sperimentale, Facoltà di Medicina, Università di Chieti, Italy.
Arterioscler Thromb. 1992 Jan;12(1):92-8. doi: 10.1161/01.atv.12.1.92.
The relation among glutathione-related enzyme activities, thiobarbituric acid-reactive substances of the human aorta and internal mammary artery, and serum lipids was studied in 40 male patients undergoing coronary revascularization. Glutathione peroxidase and glutathione reductase activities were significantly higher in the internal mammary artery, whereas glutathione transferase activity was elevated in the aortic wall. Moreover, non-selenium-dependent glutathione peroxidase activity was detectable only in the aorta. The levels of thiobarbituric acid-reactive substances were significantly higher in the aorta. A positive correlation was found among the activity of glutathione peroxidase, glutathione reductase, and thiobarbituric acid-reactive substances in the internal mammary artery and total cholesterol, low density lipoprotein cholesterol, and triglycerides. In the aortic wall, a positive correlation among the activity of glutathione peroxidase, glutathione transferase, thiobarbituric acid-reactive substances, and the previously mentioned serum lipids was evident. In contrast, high density lipoprotein cholesterol was inversely related to enzymatic activities and thiobarbituric acid-reactive substances in both the internal mammary artery and aorta. In conclusion, significant differences in the levels of glutathione-related enzyme activities and thiobarbituric acid-reactive substances in the internal mammary artery and aorta were found, suggesting a different ability of the two tissues to counteract oxidative stress: the glutathione-related antioxidant properties and the level of lipid peroxidation in the arterial tissue seem to be specifically influenced by serum lipids.
对40例接受冠状动脉血运重建术的男性患者，研究了谷胱甘肽相关酶活性、人主动脉和乳内动脉中硫代巴比妥酸反应性物质与血脂之间的关系。乳内动脉中的谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性显著更高，而主动脉壁中的谷胱甘肽转移酶活性升高。此外，非硒依赖性谷胱甘肽过氧化物酶活性仅在主动脉中可检测到。主动脉中硫代巴比妥酸反应性物质的水平显著更高。在乳内动脉中，谷胱甘肽过氧化物酶、谷胱甘肽还原酶的活性与硫代巴比妥酸反应性物质以及总胆固醇、低密度脂蛋白胆固醇和甘油三酯之间存在正相关。在主动脉壁中，谷胱甘肽过氧化物酶、谷胱甘肽转移酶的活性、硫代巴比妥酸反应性物质与上述血脂之间存在明显的正相关。相比之下，高密度脂蛋白胆固醇与乳内动脉和主动脉中的酶活性及硫代巴比妥酸反应性物质呈负相关。总之，发现乳内动脉和主动脉中谷胱甘肽相关酶活性水平和硫代巴比妥酸反应性物质存在显著差异，表明这两种组织对抗氧化应激的能力不同：动脉组织中的谷胱甘肽相关抗氧化特性和脂质过氧化水平似乎受到血脂的特异性影响。
