Zargar S, Krishnamurthi K, Saravana Devi S, Ghosh T K, Chakrabarti T
Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440 020 (MS), India.
Biomed Environ Sci. 2006 Dec;19(6):414-21.
To investigate the impact of various levels of sublethal temperature (26 degrees C, 31 degrees C, 33 degrees C, 36 degrees C, and 39 degrees C) on growth and heat shock protein (hsp) expression in freshwater green alga Scenedesmus quadricauda.
Impact of selected levels of temperature on growth rate (based on optical density), population count, chlorophyll-a and biomass of the alga was evaluated in artificial growth medium for 19 days. To determine the induction of hsp in the alga, it was exposed to selected temperature levels for 3 h and further kept for 6 h at culturing condition at 26 degrees C. Induction of hsp was confirmed by immuno-detection followed by SDS-polyacrylamide gel electrophoresis.
The selected growth parameters such as growth rate, population count, chlorophyll-a and biomass were reduced significantly (P < 0.001) at 39 degrees C. However, hsp 70 expression was observed only at 39 degrees C.
Temperature up to 36 degrees C may be considered as the limit of safe exposure for thermal stress for the alga Scenedesmus quadricauda.
研究不同亚致死温度水平（26℃、31℃、33℃、36℃和39℃）对淡水绿藻四尾栅藻生长及热休克蛋白（hsp）表达的影响。
在人工生长培养基中培养19天，评估所选温度水平对藻的生长速率（基于光密度）、种群数量、叶绿素a和生物量的影响。为确定藻中hsp的诱导情况，将其暴露于所选温度水平3小时，然后在26℃培养条件下再保持6小时。通过免疫检测和SDS-聚丙烯酰胺凝胶电泳确认hsp的诱导情况。
在39℃时，所选生长参数如生长速率、种群数量、叶绿素a和生物量显著降低（P < 0.001）。然而，仅在39℃观察到hsp 70表达。
对于四尾栅藻，36℃及以下的温度可被视为热应激安全暴露的极限。
