Hammiche Azzedine, Walsh Michael J, Pollock Hubert M, Martin-Hirsch Pierre L, Martin Francis L
Department of Physics, Lancaster University, Lancaster, UK.
J Biochem Biophys Methods. 2007 Jun 10;70(4):675-7. doi: 10.1016/j.jbbm.2007.01.011. Epub 2007 Feb 1.
We implemented a non-contact photo-thermo-mechanical recording method whereby a silicon nitride atomic force microscopy cantilever is placed several micrometer above the surface of samples. Samples were illuminated with infrared (IR) radiation after which, cantilever mechanical vibrations were optically sensed. Following spectrometric acquisition and Fourier transformation, true IR absorption spectra were obtained. With multivariate analysis, segregation between different categories of exfoliative cervical cytology was obtained. This approach points towards the implementation of a novel near-field system that allows IR spectral analysis without probe contamination.
我们实施了一种非接触式光热机械记录方法，即将氮化硅原子力显微镜悬臂置于样品表面上方几微米处。用红外（IR）辐射照射样品，然后对悬臂的机械振动进行光学传感。经过光谱采集和傅里叶变换后，获得了真实的红外吸收光谱。通过多变量分析，实现了不同类别宫颈脱落细胞学之间的区分。这种方法为一种新型近场系统的实施指明了方向，该系统可进行红外光谱分析而不会出现探针污染。
