Esmaeili Hamid Reza, Ebrahimi Mehrgan, Saifali Mahvash
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Micron. 2008;39(2):95-100. doi: 10.1016/j.micron.2006.12.007. Epub 2007 Jan 20.
The karyotypes of five tooth-carps of Iran, Aphanius persicus, Aphanius sophiae, Aphanius dispar, and Aphanius sp. have been investigated by examining metaphase chromosomes spreads obtained from gill epithelial and kidney cells. The diploid chromosome numbers of all five species were 2n=48. The karyotypes consisted of 8 pairs of submetacentric and 16 pairs of subtelocentric chromosomes in A. persicus; 4 submetacentric and 20 subtelocentric in A. sophiae; 7 submetacentric and 17 subtelocentric in Aphanius ginaonis and 8 submetacentric and 16 subtelocentric in A. dispar. It was 16 metacentric, 1 submetacentric and 7 telocentric chromosome pairs in Aphanius sp. specimen. The arm numbers were 32, 28, 31 and 32 in A. persicus, A. sophiae, A. ginaonis and A. dispar, respectively. It was 41 in Aphanius sp. Sex chromosomes were cytologically indistinguishable in these tooth-carps. Cluster analysis revealed the closeness of A. sophiae and A. persicus. According to our data A. ginaonis is related to a group of A. sophiae+A. persicus+A. dispar.
对伊朗的五种食蚊鱼，即波斯食蚊鱼（Aphanius persicus）、索菲食蚊鱼（Aphanius sophiae）、歧食蚊鱼（Aphanius dispar）和一种未明确的食蚊鱼（Aphanius sp.）的核型进行了研究，通过检查从鳃上皮细胞和肾细胞获得的中期染色体 spreads。这五个物种的二倍体染色体数均为 2n = 48。波斯食蚊鱼的核型由 8 对亚中着丝粒染色体和 16 对近端着丝粒染色体组成；索菲食蚊鱼有 4 对亚中着丝粒染色体和 20 对近端着丝粒染色体；吉氏食蚊鱼（Aphanius ginaonis）有 7 对亚中着丝粒染色体和 17 对近端着丝粒染色体；歧食蚊鱼有 8 对亚中着丝粒染色体和 16 对近端着丝粒染色体。在未明确的食蚊鱼标本中有 16 对中着丝粒染色体、1 对亚中着丝粒染色体和 7 对着丝粒染色体。波斯食蚊鱼、索菲食蚊鱼、吉氏食蚊鱼和歧食蚊鱼的臂数分别为 32、28、31 和 32。未明确的食蚊鱼的臂数为 41。在这些食蚊鱼中，性染色体在细胞学上无法区分。聚类分析显示索菲食蚊鱼和波斯食蚊鱼关系密切。根据我们的数据，吉氏食蚊鱼与索菲食蚊鱼 + 波斯食蚊鱼 + 歧食蚊鱼这一组相关。
