Murrell Joanna C, Mitchinson Sheryl L, Johnstone Alastair C, Johnson Craig B, Barnes Geoffrey R G
Institute of Veterinary Animal and Biomedical Sciences, College of Sciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
J Neurosci Methods. 2007 May 15;162(1-2):215-21. doi: 10.1016/j.jneumeth.2007.01.014. Epub 2007 Jan 25.
The study aim was to develop a model to generate cerebral evoked potentials (CEPs) by mechanical distention of the duodenum in rats. Twenty Sprague-Dawley rats were anaesthetized and the EEG recorded from the left and right somatosensory cortices (S1L, S1R). A balloon catheter was implanted into the duodenum. A pneumatic device, triggered by data acquisition software, inflated the balloon for 200 ms every 3s to deliver a repeatable noxious stimulus. EEG was recorded for 100 ms before and 500 ms after onset of inflation and the response to 512 stimuli averaged to generate a CEP. Two CEPs were generated in each animal and data summed to calculate a single CEP for each channel. Data were excluded when the signal to noise ratio was < 2, therefore data are presented from 11 animals. A repeatable CEP was identified in waveforms recorded from S1L. The mean (S.D.) CEP comprised a triphasic waveform (P1, N1, P2) with latencies of 246.0 (24.7), 289.3 (12.8) and 321.5 (13.2)ms, respectively. We are the first group to have generated and characterized a CEP following mechanical stimulation of the duodenum. This model can be applied to further elucidate the mechanisms leading to visceral pain perception.
本研究的目的是建立一种通过机械扩张大鼠十二指肠来产生脑诱发电位（CEP）的模型。将20只Sprague-Dawley大鼠麻醉，记录左、右体感皮层（S1L、S1R）的脑电图。将球囊导管植入十二指肠。一个由数据采集软件触发的气动装置每3秒使球囊充气200毫秒，以传递可重复的有害刺激。在充气开始前100毫秒和充气开始后500毫秒记录脑电图，并对512次刺激的反应进行平均以产生CEP。每只动物产生两个CEP，并将数据相加以计算每个通道的单个CEP。当信噪比<2时排除数据，因此呈现的是11只动物的数据。在从S1L记录的波形中识别出可重复的CEP。平均（标准差）CEP为三相波形（P1、N1、P2），潜伏期分别为246.0（24.7）、289.3（12.8）和321.5（13.2）毫秒。我们是第一组在十二指肠机械刺激后产生并表征CEP的研究团队。该模型可用于进一步阐明导致内脏痛觉的机制。
