Pierre Maud, Husson Marie-Odile, Le Berre Rozenn, Desseyn Jean-Luc, Galabert Claude, Béghin Laurent, Beermann Christopher, Dagenais André, Berthiaume Yves, Cardinaud Bruno, Barbry Pascal, Gottrand Frédéric, Guery Benoît P
Faculté de Médecine, Hôpital Jeanne de Flandre, Université de Lille and CHRU de Lille, France.
Am J Physiol Lung Cell Mol Physiol. 2007 Jun;292(6):L1422-31. doi: 10.1152/ajplung.00337.2006. Epub 2007 Feb 23.
Pseudomonas aeruginosa is a gram-negative bacilli frequently encountered in human pathology. This pathogen is involved in a large number of nosocomial infections and chronic diseases. Herein we investigated the effects of polyunsaturated fatty acids (PUFA) in chronic Pseudomonas aeruginosa lung infection. C57BL/6 mice were fed for 5 wk with specifically designed diets with high contents in either omega-3 (omega-3) or omega-6 PUFA and compared to a control diet. P. aeruginosa included in agarose beads was then instilled intratracheally, and the animals were studied for 7 days. On the 4th day, the mice fed with the omega-3 diet had a higher lean body mass gain and a lower omega-6:omega-3 ratio of fatty acids extracted from the lung tissue compared with the other groups (P < 0.05). The omega-3 group had the lowest mortality. Distal alveolar fluid clearance (DAFC) as well as the inflammatory response and the cellular recruitment were higher in the omega-3 group on the 4th day. The effect on DAFC was independent of alpha-epithelial Na(+) channels (alpha-ENaC), beta-ENaC, and alpha(1)-Na-K-ATPase mRNA expressions, which were not altered by the different diets. In conclusion, a diet enriched in omega-3 PUFA can change lung membrane composition and improve survival in chronic pneumonia. This effect on survival is probably multifactorial involving the increased DAFC capacity as well as the optimization of the initial inflammatory response. This work suggests that a better control of the omega-6/omega-3 PUFA balance may represent an interesting target in the prevention and/or control of P. aeruginosa infection in patients.
铜绿假单胞菌是人类病理学中常见的革兰氏阴性杆菌。这种病原体与大量医院感染和慢性疾病有关。在此，我们研究了多不饱和脂肪酸（PUFA）在慢性铜绿假单胞菌肺部感染中的作用。将C57BL/6小鼠用专门设计的富含ω-3（ω-3）或ω-6多不饱和脂肪酸的饮食喂养5周，并与对照饮食进行比较。然后将包埋在琼脂糖珠中的铜绿假单胞菌经气管内注入，并对动物进行7天的研究。在第4天，与其他组相比，用ω-3饮食喂养的小鼠瘦体重增加更高，从肺组织中提取的脂肪酸的ω-6:ω-3比值更低（P<0.05）。ω-3组的死亡率最低。在第4天，ω-3组的远端肺泡液体清除率（DAFC）以及炎症反应和细胞募集更高。对DAFC的影响独立于α-上皮钠通道（α-ENaC）、β-ENaC和α1-Na-K-ATP酶mRNA表达，这些表达不受不同饮食的影响。总之，富含ω-3多不饱和脂肪酸的饮食可以改变肺膜组成并提高慢性肺炎的生存率。这种对生存的影响可能是多因素的，涉及增加的DAFC能力以及初始炎症反应的优化。这项工作表明，更好地控制ω-6/ω-3多不饱和脂肪酸平衡可能是预防和/或控制患者铜绿假单胞菌感染的一个有趣靶点。
