Nakao Kazuhisa, Morita Ritsuko, Saji Yasumitsu, Ishida Kentaro, Tomita Yusuke, Ogawa Miho, Saitoh Masahiro, Tomooka Yasuhiro, Tsuji Takashi
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.
Nat Methods. 2007 Mar;4(3):227-30. doi: 10.1038/nmeth1012. Epub 2007 Feb 18.
To bioengineer ectodermal organs such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture method. The bioengineered tooth germ generated a structurally correct tooth, after both in vitro organ culture as well as transplantation under a tooth cavity in vivo, showing penetration of blood vessels and nerve fibers. Our method provides a substantial advance in the development of bioengineered organ replacement strategies and regenerative therapies.
为了对牙齿和触须毛囊等外胚层器官进行生物工程改造，我们开发了一种三维器官胚芽培养方法。经过体外器官培养以及在体内牙腔内移植后，生物工程改造的牙胚生成了结构正确的牙齿，显示出血管和神经纤维的长入。我们的方法在生物工程器官替代策略和再生疗法的发展方面取得了重大进展。
