Wang Wei Z, Fang Xin-Hua, Stephenson Linda L, Khiabani Kayvan T, Zamboni William A
Division of Plastic Surgery, Department of Surgery, University of Nevada School of Medicine, Las Vegas, NV 89102, USA.
Microsurgery. 2007;27(3):200-5. doi: 10.1002/micr.20331.
To determine whether the supplementation of tetrahydrobiopterin (BH(4), an essential cofactor of nitric oxide synthase; NOS) could attenuate endothelial dysfunction and improve NOS activity and cell viability in skeletal muscle after ischemia/reperfusion (I/R).
A vascular pedicle isolated rat cremaster muscle model was used. Cremaster muscles were subjected to 4 h of ischemia followed by 2 h of reperfusion. Rats were given either normal saline or BH(4) by intravenous injection at 1 min prior to reperfusion. After reperfusion, average arteriole diameter, capillary perfusion, endothelial-dependent/-independent vasodilatation, NOS activity, and muscle cell viability were evaluated.
Supplementation of BH(4) prior to reperfusion significantly attenuated reperfusion-induced vasoconstriction, poor capillary perfusion, and endothelial dysfunction and enhanced cNOS activity and slightly improved cell viability in the skeletal muscle after I/R.
Supplementation of BH(4) during reperfusion provided a significant protection against I/R injury in rat skeletal muscle.
确定补充四氢生物蝶呤（BH₄，一氧化氮合酶（NOS）的必需辅因子）是否能减轻缺血/再灌注（I/R）后骨骼肌的内皮功能障碍，提高NOS活性和细胞活力。
采用血管蒂分离大鼠提睾肌模型。提睾肌经历4小时缺血，随后2小时再灌注。在再灌注前1分钟，通过静脉注射给予大鼠生理盐水或BH₄。再灌注后，评估平均小动脉直径、毛细血管灌注、内皮依赖性/非依赖性血管舒张、NOS活性和肌肉细胞活力。
再灌注前补充BH₄可显著减轻再灌注诱导的血管收缩、毛细血管灌注不良和内皮功能障碍，并增强I/R后骨骼肌中的cNOS活性，略微改善细胞活力。
再灌注期间补充BH₄可显著保护大鼠骨骼肌免受I/R损伤。
