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[酸性矿山排水点沉积物中嗜酸菌的生物多样性]

[Biodiversity of acidophiles in the sediment at an acid mine drainage site].

作者信息

Hao Chun-Bo, Zhang Hong-Xun, Bai Zhi-Hui, Zhang Bao-Guo, Zhang Xu-Xiang

机构信息

Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

出版信息

Huan Jing Ke Xue. 2006 Nov;27(11):2255-60.

Abstract

A sediment sample was collected at an acid mine drainage site in Anhui. The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis (DGGE). The results indicated that the acidophiles in the sediment were novel compared with the data in GenBank. The acidophiles affiliated with Acidobacteria, beta/gamma-Proteobacteria, delta-Proteobacteria, Nitrospira, Candidate Division TM7, low G + C Gram-positive. delta-Proteobacteria were major group in this acidic ecosystem. The research of aicdophiles community in sediment can help us develop high efficient method to treat acid mine drainage (AMD).

摘要

在安徽的一个酸性矿山排水点采集了一份沉积物样本。利用16S rDNA克隆文库和变性梯度凝胶电泳(DGGE)研究了沉积物中的嗜酸微生物组成和群落结构。结果表明,与GenBank中的数据相比,沉积物中的嗜酸微生物是新发现的。嗜酸微生物隶属于酸杆菌门、β/γ-变形菌纲、δ-变形菌纲、硝化螺旋菌门、候选分类群TM7、低G + C革兰氏阳性菌。δ-变形菌纲是这个酸性生态系统中的主要类群。对沉积物中嗜酸微生物群落的研究有助于我们开发高效的酸性矿山排水(AMD)处理方法。

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