垂体腺苷酸环化酶激活多肽刺激离体灌流大鼠胰腺释放胰岛素。
Pituitary adenylate cyclase activating polypeptide stimulates insulin release from the isolated perfused rat pancreas.
作者信息
Kawai K, Ohse C, Watanabe Y, Suzuki S, Yamashita K, Ohashi S
机构信息
Department of Internal Medicine, University of Tsukuba, Ibaraki-ken, Japan.
出版信息
Life Sci. 1992;50(4):257-61. doi: 10.1016/0024-3205(92)90332-j.
Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide structurally related to vasoactive intestinal peptide (VIP) and glucagon like peptide-1(7-36) amide (tGLP-1) in its N-terminal portion. Therefore, their levels of insulinotropic potency were compared using an isolated rat pancreas perfusion. It was found that 0.1 nM PACAP (1-27) amide (PACAP27) significantly stimulated insulin release under a perfusate glucose concentration of 5.5 mM, whereas 1 nM PACAP27 did not under a perfusate glucose concentration of 2.8 mM. The potency was evaluated as tGLP-1 greater than PACAP27 greater than VIP. These results indicate that PACAP is a glucagon superfamily peptide which stimulates insulin release in a glucose dependent manner.
垂体腺苷酸环化酶激活多肽（PACAP）是一种新型的下丘脑肽，其N端部分在结构上与血管活性肠肽（VIP）和胰高血糖素样肽-1（7-36）酰胺（tGLP-1）相关。因此，使用离体大鼠胰腺灌注比较了它们的促胰岛素分泌能力。结果发现，在灌注液葡萄糖浓度为5.5 mM时，0.1 nM PACAP（1-27）酰胺（PACAP27）能显著刺激胰岛素释放，而在灌注液葡萄糖浓度为2.8 mM时，1 nM PACAP27则不能。其效力评估为tGLP-1大于PACAP27大于VIP。这些结果表明，PACAP是一种以葡萄糖依赖方式刺激胰岛素释放的胰高血糖素超家族肽。
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