Gogberashvili K Ia
Georgian Med News. 2007 Jan(142):70-2.
Study was designed to show the affects of short-term intermittent and/or continuous maternal separation on norepinephrine and dopamine blood concentrations among rat pups. The 128 rat pups on the first, 15th, 21st, and 60th days after birth were investigated. Catheholamins blood concentration were measured by spectral fluorometry. The pathological changes in norepinephrine (NE) and dopamine (DA) plasma concentrations were exposed in both occasions - among rat pups with intermittent as well as with continuous maternal separation but were less pronounced in rat pups exposed to permanent maternal deprivation. The intermittent maternal separation produced a significant increase in plasma NE concentration compared to control non-deprived (p<0,001) and also to permanently deprived rats (p<0,01). While DA concentration, instead of elevation, was decreased in deprived rats. It was significantly lower in continuously deprived animals. The accentuated increase of NE suggests sympathetic nervous system hyperactivity, while decrease of DA indicates discoordination of the dopaminergic and sympathoadrenal systems in deprived pups. Thus, early maternal separation in rat pups may serve to be the stressful stimulus for CNS and alter it function, which produces the behavioral disorders among them.
本研究旨在探讨短期间歇性和/或持续性母婴分离对幼鼠血液中去甲肾上腺素和多巴胺浓度的影响。对出生后第1天、第15天、第21天和第60天的128只幼鼠进行了研究。采用光谱荧光法测定儿茶酚胺类血液浓度。在间歇性和持续性母婴分离的幼鼠中，去甲肾上腺素（NE）和多巴胺（DA）血浆浓度均出现了病理变化，但在永久性母婴剥夺的幼鼠中变化不太明显。与未剥夺的对照幼鼠相比（p<0.001），以及与永久性剥夺的幼鼠相比（p<0.01），间歇性母婴分离使血浆NE浓度显著升高。而在剥夺幼鼠中，DA浓度非但升高，反而降低。在持续性剥夺的动物中，DA浓度显著更低。NE的显著升高提示交感神经系统功能亢进，而DA的降低表明剥夺幼鼠中多巴胺能系统和交感肾上腺系统失调。因此，幼鼠早期母婴分离可能是中枢神经系统的应激源，并改变其功能，进而导致幼鼠出现行为障碍。
