Fujikawa Shigenori, Muto Emi, Kunitake Toyoki
Topochemical Design Laboratory, and Innovative Nanopatterning Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
Langmuir. 2007 Apr 10;23(8):4629-33. doi: 10.1021/la0635247. Epub 2007 Mar 1.
We report herein the fabrication of ferritin-embedded self-supporting silica nanofilms via a simple spin-coating process. Ferritin was employed as a template molecule, and solutions of ferritin and silica were spread on a polymer-coated silicon substrate, in this order. After dissolving the polymer underlayer by simply immersing ethanol, a centimeter-sized self-supporting nanofilm of ferritin/silica composite with a thickness of 15 nm was successfully transferred onto an alumina membrane without the film breaking. Ozone and hydrochloric acid solution treatment removed the template ferritin molecules from the composite film to produce corresponding transmembrane nanoholes. The reported method is very simple, and the fabrication of a protein-embedded self-supporting nanofilm enables the design of biomembrane-mimetic devices.
我们在此报告通过简单的旋涂工艺制备铁蛋白嵌入的自支撑二氧化硅纳米薄膜。铁蛋白被用作模板分子，铁蛋白溶液和二氧化硅溶液依次铺展在聚合物涂层的硅基板上。通过简单地浸入乙醇溶解聚合物底层后，成功地将厚度为15nm、厘米大小的铁蛋白/二氧化硅复合材料自支撑纳米薄膜转移到氧化铝膜上而薄膜未破裂。臭氧和盐酸溶液处理从复合膜中去除模板铁蛋白分子以产生相应的跨膜纳米孔。所报道的方法非常简单，并且蛋白质嵌入的自支撑纳米薄膜的制备能够设计仿生膜装置。
