• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
C-Npys (S-3-nitro-2-pyridinesulfenyl) and peptide derivatives can inhibit a serine-thiol proteinase activity from Paracoccidioides brasiliensis.C-Npys（S-3-硝基-2-吡啶基亚磺酰基）及其肽衍生物能够抑制巴西副球孢子菌的一种丝氨酸-硫醇蛋白酶活性。
Biochem Biophys Res Commun. 2007 Apr 20;355(4):1000-5. doi: 10.1016/j.bbrc.2007.02.070. Epub 2007 Feb 23.
2
Modulation of the exocellular serine-thiol proteinase activity of Paracoccidioides brasiliensis by neutral polysaccharides.
Microbes Infect. 2006 Jan;8(1):84-91. doi: 10.1016/j.micinf.2005.05.021. Epub 2005 Aug 8.
3
Characterization of an exocellular serine-thiol proteinase activity in Paracoccidioides brasiliensis.巴西副球孢子菌胞外丝氨酸 - 硫醇蛋白酶活性的特性研究
Biochem J. 1995 Jul 1;309 ( Pt 1)(Pt 1):209-14. doi: 10.1042/bj3090209.
4
Exocellular proteolytic activity of Paracoccidioides brasiliensis: cleavage of components associated with the basement membrane.
Med Mycol. 1998 Oct;36(5):345-8.
5
Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp.
Braz J Med Biol Res. 1999 May;32(5):645-9. doi: 10.1590/s0100-879x1999000500019.
6
The 3-nitro-2-pyridinesulfenyl group: synthesis and applications to peptide chemistry.3-硝基-2-吡啶亚磺酰基：合成及其在肽化学中的应用
J Pept Sci. 2017 Jul;23(7-8):496-504. doi: 10.1002/psc.2964. Epub 2017 Jan 25.
7
Effects of sulfhydryl-modifying reagents, 3-nitro-2-pyridinesulfenyl compounds, on the coupling between inhibitory receptors and GTP-binding proteins Gi/Go in rat brain membranes.巯基修饰试剂3-硝基-2-吡啶硫基化合物对大鼠脑膜中抑制性受体与GTP结合蛋白Gi/Go偶联的影响。
Mol Pharmacol. 1990 Aug;38(2):184-91.
8
3-nitro-2-pyridinesulfenyl (Npys) group. A novel selective protecting group which can be activated for peptide bond formation.3-硝基-2-吡啶亚磺酰基（Npys）基团。一种新型的选择性保护基团，可被激活用于肽键形成。
Int J Pept Protein Res. 1980 Nov;16(5):392-401.
9
Thiolysis of the 3-nitro-2-pyridinesulfenyl (Npys) protecting group. An approach towards a general deprotection scheme in peptide synthesis.3-硝基-2-吡啶亚磺酰基（Npys）保护基的硫解。一种肽合成中通用脱保护方案的方法。
Int J Pept Protein Res. 1990 Jun;35(6):545-9. doi: 10.1111/j.1399-3011.1990.tb00260.x.
10
Cyclic, linear, cycloretro-isomer, and cycloretro-inverso peptides derived from the C-terminal sequence of bradykinin as substrates or inhibitors of serine and cysteine proteases.源自缓激肽C末端序列的环状、线性、环逆异构和环逆反式肽作为丝氨酸和半胱氨酸蛋白酶的底物或抑制剂。
Protein J. 2004 May;23(4):287-94. doi: 10.1023/b:jopc.0000027853.93513.34.

引用本文的文献

1
The story of Paracoccidiodes gp43.球腔菌 gp43 的故事。
Braz J Microbiol. 2023 Dec;54(4):2543-2550. doi: 10.1007/s42770-023-00962-y. Epub 2023 Apr 13.

本文引用的文献

1
Modulation of the exocellular serine-thiol proteinase activity of Paracoccidioides brasiliensis by neutral polysaccharides.
Microbes Infect. 2006 Jan;8(1):84-91. doi: 10.1016/j.micinf.2005.05.021. Epub 2005 Aug 8.
2
Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells.巴西副球孢子菌在菌丝体和酵母细胞中的转录谱。
J Biol Chem. 2005 Jul 1;280(26):24706-14. doi: 10.1074/jbc.M500625200. Epub 2005 Apr 22.
3
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: identification of putative homologues of Candida albicans virulence and pathogenicity genes.巴西副球孢子菌酵母相的表达序列标签分析：白色念珠菌毒力和致病基因假定同源物的鉴定
Eukaryot Cell. 2003 Feb;2(1):34-48. doi: 10.1128/EC.2.1.34-48.2003.
4
Secreted proteases from pathogenic fungi.致病真菌分泌的蛋白酶。
Int J Med Microbiol. 2002 Oct;292(5-6):405-19. doi: 10.1078/1438-4221-00223.
5
Paracoccidioides brasiliensis and paracoccidioidomycosis: molecular approaches to morphogenesis, diagnosis, epidemiology, taxonomy and genetics.巴西副球孢子菌与副球孢子菌病：形态发生、诊断、流行病学、分类学及遗传学的分子研究方法
Med Mycol. 2002 Jun;40(3):225-42. doi: 10.1080/mmy.40.3.225.242.
6
Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis.用于使用基质辅助激光解吸/电离飞行时间分析进行高灵敏度蛋白质鉴定的改进银染方案。
Proteomics. 2001 Nov;1(11):1359-63. doi: 10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q.
7
Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp.
Braz J Med Biol Res. 1999 May;32(5):645-9. doi: 10.1590/s0100-879x1999000500019.
8
Exocellular proteolytic activity of Paracoccidioides brasiliensis: cleavage of components associated with the basement membrane.
Med Mycol. 1998 Oct;36(5):345-8.
9
Subtilases: the superfamily of subtilisin-like serine proteases.枯草杆菌蛋白酶：枯草杆菌蛋白酶样丝氨酸蛋白酶超家族
Protein Sci. 1997 Mar;6(3):501-23. doi: 10.1002/pro.5560060301.
10
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.银染聚丙烯酰胺凝胶的蛋白质质谱测序
Anal Chem. 1996 Mar 1;68(5):850-8. doi: 10.1021/ac950914h.

C-Npys（S-3-硝基-2-吡啶基亚磺酰基）及其肽衍生物能够抑制巴西副球孢子菌的一种丝氨酸-硫醇蛋白酶活性。

C-Npys (S-3-nitro-2-pyridinesulfenyl) and peptide derivatives can inhibit a serine-thiol proteinase activity from Paracoccidioides brasiliensis.

作者信息

Matsuo Alisson L, Carmona Adriana K, Silva Luiz S, Cunha Carlos E L, Nakayasu Ernesto S, Almeida Igor C, Juliano Maria A, Puccia Rosana

机构信息

Department of Microbiology, Immunology and Parasitology, Cell Biology Division, Federal University of São Paulo, São Paulo, SP 04023-062, Brazil.

出版信息

Biochem Biophys Res Commun. 2007 Apr 20;355(4):1000-5. doi: 10.1016/j.bbrc.2007.02.070. Epub 2007 Feb 23.

DOI:10.1016/j.bbrc.2007.02.070
PMID:17328865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3606896/
Abstract

The inhibitory capacity of C-Npys (S-[3-nitro-2-pyridinesulfenyl]) derivatives over thiol-containing serine proteases has never been tested. In the present work we used an extracellular serine-thiol proteinase activity from the fungal pathogen Paracoccidioides brasiliensis (PbST) to describe a potent inhibitory capacity of Bzl-C(Npys)KRLTL-NH(2) and Bzl-MKRLTLC(Npys)-NH(2). The assays were performed with PbST enriched upon affinity chromatography in a p-aminobenzamidine (pABA)-Sepharose column. Although PbST can cleave the fluorescence resonance energy transfer peptide Abz-MKRLTL-EDDnp between L-T, the C(Npys) derivatives were not substrates nor were they toxic in a cell detachment assay, allowing therapeutic use. The best inhibitor was Bzl-C(Npys)KRLTL-NH(2) (K(i)=16nM), suggesting that the peptide sequence promoted a favorable interaction, especially when C(Npys) was placed at a further position from the L-T bond, at the N-terminus. Inhibition was completely reverted with dithioerythritol, indicating that it was due to the reactivity of the C(Npys) moiety with a free SH- group.

摘要

C-Npys（S-[3-硝基-2-吡啶基亚磺酰基]）衍生物对含硫醇的丝氨酸蛋白酶的抑制能力从未被测试过。在本研究中，我们利用巴西副球孢子菌（PbST）的一种细胞外丝氨酸-硫醇蛋白酶活性来描述Bzl-C（Npys）KRLTL-NH(2)和Bzl-MKRLTLC（Npys）-NH(2)的强大抑制能力。实验是用在对氨基苯甲脒（pABA）-琼脂糖柱上经亲和层析富集的PbST进行的。尽管PbST可以在L-T之间切割荧光共振能量转移肽Abz-MKRLTL-EDDnp，但C（Npys）衍生物既不是底物，在细胞脱离实验中也没有毒性，从而允许用于治疗。最佳抑制剂是Bzl-C（Npys）KRLTL-NH(2)（K(i)=16nM），这表明该肽序列促进了一种有利的相互作用，特别是当C（Npys）位于离L-T键更远的N端位置时。用二硫苏糖醇可完全逆转抑制作用，表明这是由于C（Npys）部分与游离SH基团的反应性所致。