一种改进的用于检测禾谷缢管蚜病毒（RhPV）的方法能够监测其在蚜虫体内的存在情况以及在植物体内的移动情况。
An improved detection method for the Rhopalosiphum padi virus (RhPV) allows monitoring of its presence in aphids and movement within plants.
作者信息
Ban L, Didon A, Jonsson L M V, Glinwood R, Delp G
机构信息
Department of Ecology, Swedish University of Agricultural Sciences, Box 7044, SE-750 07 Uppsala, Sweden.
出版信息
J Virol Methods. 2007 Jun;142(1-2):136-42. doi: 10.1016/j.jviromet.2007.01.014. Epub 2007 Feb 27.
Rhopalosiphum padi virus (RhPV) is an insect RNA virus that infects aphids, reducing their lifespan and fecundity. It can be transmitted vertically between aphids and horizontally via the plant. An improved detection method for the virus in aphids and plants using RT-PCR was developed; this allowed individual aphids to be tested for RhPV. Testing of R. padi aphids collected from different sites in Sweden revealed the presence of RhPV in wild aphid populations for the first time in Europe. Virus could be detected in several life stages of R. padi, including sexual individuals and eggs, establishing an over-wintering route for the virus. Using RT-PCR, systemic transport of the virus in plants was tracked. Virus spread from the aphid feeding site to all parts of the plant, including roots, within 7 days, and could be acquired by virus-free aphids feeding on the same plant.
禾谷缢管蚜病毒（RhPV）是一种感染蚜虫的昆虫RNA病毒，会缩短蚜虫的寿命并降低其繁殖力。它可以在蚜虫之间垂直传播，也可以通过植物进行水平传播。开发了一种使用逆转录聚合酶链反应（RT-PCR）在蚜虫和植物中检测该病毒的改进方法；这使得能够对单个蚜虫进行RhPV检测。对从瑞典不同地点采集的禾谷缢管蚜进行检测，首次在欧洲野生蚜虫种群中发现了RhPV。在禾谷缢管蚜的几个生命阶段都能检测到病毒，包括有性个体和卵，从而确定了病毒的越冬途径。使用RT-PCR追踪了病毒在植物中的系统运输。病毒在7天内从蚜虫取食部位传播到植物的所有部位，包括根部，并且可以被取食同一植物的无病毒蚜虫获取。
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