Crane Yan M, Crane Charles F, Subramanyam Subhashree, Schemerhorn Brandon J
Crop Production and Pest Control Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), West Lafayette, IN 47907, USA.
Department of Entomology, Purdue University, West Lafayette, IN 47907, USA.
Insects. 2025 May 23;16(6):554. doi: 10.3390/insects16060554.
The greenbug aphid ( (Rondani)) is a major pest of wheat and an important vector of wheat viruses. An RNA-seq study was conducted to investigate the microbial effects of two greenbug genotypes, the presence or absence of cereal yellow dwarf virus, and the condition of the wheat host over a 20-day time course of unrestricted greenbug feeding. Messenger RNA reads were mapped to ca. 47,000 bacterial, 1218 archaeal, 14,165 viral, 571 fungal, and 94 protozoan reference or representative genomes, plus greenbug itself and its wheat host. Taxon counts were analyzed with QIIME2 and DESeq2. Distinct early (days 1 through 10) and late (days 15 and 20) communities differed in the abundance of typical enteric genera (, , ), which declined in the late community, while the ratio of microbial to greenbug read counts declined 50% and diversity measures increased. The nearly universal aphid endosymbiont, , accounted for less than 25% of the read counts in both communities. There were 302 differentially expressed (populated) genera with respect to early and late dates, while 25 genera differed between the greenbug genotypes and nine differed between carrier and virus-free greenbugs. The late community was likely responding to starvation as the wheat host succumbed to aphid feeding. Our results add to basic knowledge about aphid microbiomes and offer an attractive alternative method to assess insect microbiomes.
麦二叉蚜（Rondani）是小麦的主要害虫以及小麦病毒的重要传播媒介。开展了一项RNA测序研究，以调查在20天无限制麦二叉蚜取食的时间进程中，两种麦二叉蚜基因型、是否存在小麦黄矮病毒以及小麦宿主状况的微生物效应。信使RNA读数被映射到约47,000个细菌、1218个古菌、14,165个病毒、571个真菌和94个原生动物的参考或代表性基因组，以及麦二叉蚜自身及其小麦宿主。使用QIIME2和DESeq2分析分类群计数。不同的早期（第1天至第10天）和晚期（第15天和第20天）群落中典型肠道属（如 、 、 ）的丰度存在差异，这些属在晚期群落中减少，而微生物与麦二叉蚜读数计数的比率下降了50%，多样性指标增加。几乎普遍存在的蚜虫内共生菌 在两个群落中的读数计数均占不到25%。在早期和晚期之间有302个差异表达（有种群）的属，而在麦二叉蚜基因型之间有25个属不同，在携带病毒和无病毒的麦二叉蚜之间有9个属不同。随着小麦宿主因蚜虫取食而死亡，晚期群落可能是对饥饿做出反应。我们的结果增加了关于蚜虫微生物组的基础知识，并提供了一种有吸引力的评估昆虫微生物组的替代方法。
