Wu Yihang, Yang Leixiang, Wang Fang, Wu Xiumei, Zhou Changxin, Shi Shuyun, Mo Jianxia, Zhao Yu
Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Food Chem Toxicol. 2007 Aug;45(8):1349-55. doi: 10.1016/j.fct.2007.01.011. Epub 2007 Jan 24.
Although Laggera pterodonta as a folk medicine has been widely used for several centuries to ameliorate some inflammatory ailments as hepatitis in China, there have been no studies of the hepatoprotective and antioxidative effects of this plant. In this paper, the hepatoprotective effect of total phenolics from L. pterodonta (TPLP) against CCI4-, D-GalN-, TAA-, and t-BHP-induced injury was examined in primary cultured neonatal rat hepatocytes. TPLP inhibited the cellular leakage of two enzymes, hepatocyte ASAT and ALAT, caused by these chemicals and improved cell viability. Moreover, TPLP afforded much stronger protection than the reference drug silibinin. Meanwhile, DPPH and superoxide radicals scavenging activities of TPLP were also determined. The present investigation is the first to report chemical-induced injury model in primary cultured neonatal rat hepatocytes and provide evidence for the hepatoprotective and antioxidative effects of L. pterodonta. Neutralizing reactive oxygen species by nonenzymatic mechanisms may be one of main mechanisms of TPLP against chemical-induced hepatocyte injury. Furthermore, The total phenolic content of L. pterodonta and its main component type were quantified, and its principle components isochlorogenic acids were isolated and authenticated. These data support the folkloric uses of L. pterodonta in the treatment of hepatitis.
尽管作为一种民间药物，翼齿六棱菊在中国已被广泛用于缓解一些炎症性疾病如肝炎达几个世纪之久，但尚未有关于该植物保肝和抗氧化作用的研究。本文在原代培养的新生大鼠肝细胞中检测了翼齿六棱菊总酚（TPLP）对四氯化碳、D-半乳糖胺、硫代乙酰胺和叔丁基过氧化氢诱导损伤的保肝作用。TPLP抑制了这些化学物质引起的两种酶即肝细胞天冬氨酸氨基转移酶和丙氨酸氨基转移酶的细胞渗漏，并提高了细胞活力。此外，TPLP提供的保护作用比参比药物水飞蓟宾强得多。同时，还测定了TPLP对二苯基苦味酰基自由基和超氧阴离子自由基的清除活性。本研究首次报道了原代培养新生大鼠肝细胞中的化学诱导损伤模型，并为翼齿六棱菊的保肝和抗氧化作用提供了证据。通过非酶机制中和活性氧可能是TPLP对抗化学诱导肝细胞损伤的主要机制之一。此外，对翼齿六棱菊的总酚含量及其主要成分类型进行了定量，并分离鉴定了其主要成分异绿原酸。这些数据支持了翼齿六棱菊在治疗肝炎方面的民间应用。
