Hsieh Bo-Chuan, Matsumoto Kiyoshi, Cheng Tzong-Jih, Yuu Gyokurin, Chen Richie L C
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, 136 Chou-Shan Road, Taipei City 106, Taiwan, ROC.
J Pharm Biomed Anal. 2007 Nov 30;45(4):673-8. doi: 10.1016/j.jpba.2007.01.031. Epub 2007 Jan 24.
An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (<50.0 microm in thickness) was covalently immobilized with choline oxidase (EC 3.1.1.17 from Alcaligenes sp.) and then attached onto the platinum electrode of an amperometric flow cell. The flow cell served as the choline sensing device of the proposed FIA system. The sensor signal (peak height of the FIAgram) was linearly related to choline concentration (r=0.999 for choline up to 5.0mM) with low detection limit (S/N>3 for 10.0 microM choline) and high reproducibility (CV<3% for 1.0mM choline, n=7). The system was proved to be useful in measuring cholinesterase inhibitory activities of synthetic chemicals or natural products.
利用天然几丁质膜作为固定生物催化剂的支撑材料，组装了一种安培型流动注射胆碱生物传感器，该膜是从台湾招潮蟹（Mictyris brevidactylus）中提纯得到的。将几丁质膜（厚度小于50.0微米）与胆碱氧化酶（来自产碱菌属的EC 3.1.1.17）共价固定，然后附着在安培流动池的铂电极上。该流动池用作所提出的流动注射分析（FIA）系统的胆碱传感装置。传感器信号（FIA图的峰高）与胆碱浓度呈线性关系（胆碱浓度高达5.0 mM时r = 0.999），检测限低（10.0 microM胆碱时S/N>3）且重现性高（1.0 mM胆碱时CV<3%，n = 7）。该系统被证明可用于测量合成化学品或天然产物的胆碱酯酶抑制活性。
