大鼠肝脏中超氧化物歧化酶的亚细胞定位
Subcellular localization of superoxide dismutase in rat liver.
作者信息
Peeters-Joris C, Vandevoorde A M, Baudhuin P
出版信息
Biochem J. 1975 Jul;150(1):31-9. doi: 10.1042/bj1500031.
The subcellular localization of superoxide dismutase was investigated in rat liver homogenates. Most of the superoxide dismutase activity is present in the soluble fraction (84%), the rest being associated with mitochondria. No indications for the occurrence of superoxide dismutase in other subcellular structures, particularly in peroxisomes, was found. Mitochondrial activity is not due to adsorption, since the sedimentable activity is essentially latent. Subfractionation of mitochondria by hypo-osmotic shock and sonication shows that half of the mitochondrial superoxide dismutase activity is localized in the intermembrane space, the rest of the enzyme being a component of the matrix space. In non-ionic media the matrix enzyme is, however, adsorbed to the inner membrane, from which it can be desorbed by low (0.04M) concentration of KCl. Superoxide dismutase activity was found in all rat organs investigated. Maximal activity of the enzyme is observed in liver, adrenals and kidney. In adrenals, the highest specific activity is associated with the medulla.
对大鼠肝脏匀浆中超氧化物歧化酶的亚细胞定位进行了研究。大部分超氧化物歧化酶活性存在于可溶部分（84%），其余与线粒体相关。未发现超氧化物歧化酶在其他亚细胞结构中存在的迹象，特别是在过氧化物酶体中。线粒体活性并非由于吸附，因为可沉降活性基本上是潜在的。通过低渗休克和超声处理对线粒体进行亚分级分离表明，线粒体超氧化物歧化酶活性的一半位于膜间隙，其余的酶是基质空间的一个组成部分。然而，在非离子介质中，基质酶会吸附在内膜上，低浓度（0.04M）的氯化钾可使其从内膜上解吸。在所研究的所有大鼠器官中均发现了超氧化物歧化酶活性。该酶在肝脏、肾上腺和肾脏中活性最高。在肾上腺中，最高的比活性与髓质相关。
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