Paul L C, Grothman G T, Benediktsson H, Davidoff A, Rozing J
Department of Medicine, University of Calgary, Canada.
Transplantation. 1992 Jan;53(1):157-62. doi: 10.1097/00007890-199201000-00032.
The purpose of the present study was to investigate the phenotype of macrophages that infiltrate normal and transplanted rat tissues. The macrophage monoclonal antibodies ED1, ED2, ED3, 52-1D4, ER15, and OX43, together with antibodies against lymphocyte and class II MHC antigens, were used in an indirect immunofluorescence technique with sections of normal tissues and heart and renal grafts that experienced long-term survival or rejection. A small number of ED1- and ED3-positive interstitial cells were detected in normal heart and renal tissues and their number increased dramatically in rejection. Normal heart tissue contained a population of ED2-positive cells with dendritic morphology that was not detected in renal tissue. Following transplantation, a diffuse increase of rounded ED2-positive cells was observed in heart grafts; no ED2-positive cells were detected in grafts removed after 20-30 days from nonimmunosuppressed recipients. Grafts from CsA-treated animals or grafts that survived greater than 50 days in nonimmunosuppressed recipients exhibited the interstitial dendritic pattern of ED2-positive cells. Only very few rounded ED2-positive cells were observed in renal allografts; if present, they were mostly located in the medulla. OX43, which bound in normal tissues to vessel endothelium and a population of macrophages, stained in allografts an additional small population of graft-infiltrating cells, and in F344 renal allografts a population of multinucleated giant cells. We conclude that the posttransplant macrophage infiltration pattern of heart and renal allografts, defined by the monocyte/macrophage antibodies ED1, ED3, 52-1D4, and ER15, is very similar for both types of organs, although the antibody ED2 and the endothelial-macrophage antibody OX43 revealed remarkable differences between the two types of organ allografts.
本研究的目的是调查浸润正常和移植大鼠组织的巨噬细胞表型。巨噬细胞单克隆抗体ED1、ED2、ED3、52-1D4、ER15和OX43，以及抗淋巴细胞和II类MHC抗原的抗体，被用于对正常组织、心脏和长期存活或发生排斥反应的肾移植组织切片进行间接免疫荧光技术检测。在正常心脏和肾组织中检测到少量ED1和ED3阳性的间质细胞，在排斥反应时其数量显著增加。正常心脏组织含有一群具有树突形态的ED2阳性细胞，而在肾组织中未检测到。移植后，在心脏移植物中观察到圆形ED2阳性细胞弥漫性增加；在未免疫抑制受体的移植物于20-30天后切除时未检测到ED2阳性细胞。来自环孢素处理动物的移植物或在未免疫抑制受体中存活超过50天的移植物呈现出ED2阳性细胞的间质树突状模式。在肾同种异体移植物中仅观察到极少数圆形ED2阳性细胞；若存在，它们大多位于髓质。OX43在正常组织中与血管内皮和一群巨噬细胞结合，在同种异体移植物中还对另一小群移植物浸润细胞染色，在F344肾同种异体移植物中对一群多核巨细胞染色。我们得出结论，由单核细胞/巨噬细胞抗体ED1、ED3、52-1D4和ER15定义的心脏和肾同种异体移植物移植后巨噬细胞浸润模式在两种器官类型中非常相似，尽管抗体ED2和内皮巨噬细胞抗体OX43揭示了两种器官同种异体移植物之间的显著差异。
