在大西洋庸鲽幼鱼养殖设施的海水中检测诺达病毒。
Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae.
作者信息
Nerland Audun H, Skaar Cecilie, Eriksen Tove Boge, Bleie Hogne
机构信息
Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway.
出版信息
Dis Aquat Organ. 2007 Jan 18;73(3):201-5. doi: 10.3354/dao073201.
We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 x 10(4) TCID50 (50% tissue culture infectious dose) ml(-1). Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 x 10(7) virus particles ml(-1) seawater.
我们采用（1）超速离心后进行逆转录聚合酶链反应（RT-PCR）以及（2）实时逆转录聚合酶链反应（real-time RT-PCR）来检测和定量养殖设施中饲养大西洋庸鲽（Hippoglossus hippoglossus）幼体/鱼苗的海水中的诺达病毒。与体外增殖病毒相比，病毒浓度相当于1.6×10⁴半数组织培养感染剂量（TCID50）ml⁻¹。与体外转录RNA相比，浓度估计为2×10⁷病毒颗粒ml⁻¹海水。
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