Nerland Audun H, Skaar Cecilie, Eriksen Tove Boge, Bleie Hogne
Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway.
Dis Aquat Organ. 2007 Jan 18;73(3):201-5. doi: 10.3354/dao073201.
We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 x 10(4) TCID50 (50% tissue culture infectious dose) ml(-1). Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 x 10(7) virus particles ml(-1) seawater.
我们采用（1）超速离心后进行逆转录聚合酶链反应（RT-PCR）以及（2）实时逆转录聚合酶链反应（real-time RT-PCR）来检测和定量养殖设施中饲养大西洋庸鲽（Hippoglossus hippoglossus）幼体/鱼苗的海水中的诺达病毒。与体外增殖病毒相比，病毒浓度相当于1.6×10⁴半数组织培养感染剂量（TCID50）ml⁻¹。与体外转录RNA相比，浓度估计为2×10⁷病毒颗粒ml⁻¹海水。
