Zhang Bei-lei, Yang Zhi-hui, Ran Peng, Liang Wei-bo, Zhou Bin, Zhang Geng-qian, Lu Mei-li, Zhang Lin
Department of Forensic Biology, West China School of Preclinical and Forensic medicine, Sichuan Univesity, Chengdu 610041, China.
Fa Yi Xue Za Zhi. 2007 Feb 15;23(1):4-7.
To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia.
Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei.
HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.
研究缺氧诱导因子1-α（HIF1-α）在两种典型窒息模型死亡大鼠的心、肺、肝和肾中的表达情况。
制作两种窒息模型，在不同的死后时间从不同组收集死亡大鼠的组织样本。采用免疫组织化学和图像分析技术检测各组织中HIF1-α的表达及变化。结果在两种窒息模型中，均观察到心肌纤维、肾细胞、肝细胞和肺细胞中有HIF1-α的显著表达，而对照组中未观察到。在氮气吸入致死的大鼠中，各组织在0小时时HIF1-α表达于细胞核，此后表达水平逐渐下降。缢死大鼠各组织中HIF1-α的表达水平和持续时间均高于前一组且持续时间更长，在死后6小时观察到表达水平达到峰值，然后除心脏外所有组织的表达开始下降，心脏在死后24小时表达仍呈上升趋势。对照组在细胞质中有稳定表达，但细胞核中无表达。
HIF1-α似乎是死后24小时内窒息诊断中有价值的生物标志物。
