Liu Xiao-shan, Zang Lin-quan, Hao Zi-rui, Li Zhao-hui, Liu Shui-ping, Chen Yu-chuan, Qu Jing-dong
Faculty of Forensic Medicine, Preclinical School of SUN Yat-sen University, Guangzhou 510080, China.
Fa Yi Xue Za Zhi. 2007 Feb 15;23(1):14-7.
To investigate whether heroin can directly induce apoptosis in primary cultured cortical neurons of rat's brain.
Cultured primary neurons cultures were obtained from cerebral cortex of embryo rats. After 7 days, the cells were incubated with different concentrations of heroin (purity-80%) for 24 hours. The neuronal survival was assessed by cell viability counting with fluorescent diacetate (FDA) staining. The morphological and biochemical changes were observed with Hoechst 33258 fluorescent staining and then analyzed by agarose gel electrophoresis, respectively.
After treatment with different concentrations of heroin, the neurons showed a decreased survival rate in a dose dependent manner, and there was a significant difference in the survival rate between the heroin group and the control group (P < 0.05). When exposed to different concentrations of heroin, neurons exhibited the morphological and biochemical features of apoptosis, including cell shrinkage, neurite degeneration, network disappearance, condensation and aggregation of nuclear chromatin, and the formation of DNA ladders. With the increase of heroin concentration of rat's brain more apoptotic bodies were seen.
Heroin can directly induce apoptosis in primary cultured cortical neurons in rat's brain.
研究海洛因是否能直接诱导大鼠脑原代培养皮层神经元凋亡。
从胚胎大鼠大脑皮层获取原代神经元培养物。7天后，将细胞与不同浓度的海洛因（纯度80%）孵育24小时。通过荧光双醋酸酯（FDA）染色进行细胞活力计数来评估神经元存活情况。分别用Hoechst 33258荧光染色观察形态学和生化变化，然后进行琼脂糖凝胶电泳分析。
用不同浓度海洛因处理后，神经元存活率呈剂量依赖性降低，海洛因组与对照组存活率之间存在显著差异（P<0.05）。当暴露于不同浓度海洛因时，神经元呈现出凋亡的形态学和生化特征，包括细胞皱缩、神经突退化、网络消失、核染色质凝聚和聚集以及DNA梯带形成。随着大鼠脑海洛因浓度增加，可见更多凋亡小体。
海洛因可直接诱导大鼠脑原代培养皮层神经元凋亡。
