Findlow Helen, Vogel Ulrich, Mueller Judith E, Curry Alan, Njanpop-Lafourcade Berthe-Marie, Claus Heike, Gray Stephen J, Yaro Seydou, Traoré Yves, Sangaré Lassana, Nicolas Pierre, Gessner Bradford D, Borrow Ray
Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Royal Infirmary, Manchester, M13 9WZ, UK.
J Infect Dis. 2007 Apr 1;195(7):1071-7. doi: 10.1086/512084. Epub 2007 Feb 16.
During reinforced surveillance of acute bacterial meningitis in Burkina Faso, meningococcal strains of phenotype NG:NT:NST were isolated from cerebrospinal fluid samples from 3 patients. The strains were negative for the ctrA gene but were positive for the crgA gene. Molecular typing revealed that the strains harbored the capsule null locus (cnl) and belonged to the multilocus sequence type (ST)-192. PorA sequencing showed that all strains were either P1.18-11,42; P1.18,42-1; P1.18-11,42-1; P1.18-11,42-3; or P1.18-12,42-1. Sequencing also showed that all strains were negative for the FetA receptor gene. Serum killing assays showed these strains to be resistant, with the resistance comparable with that of a fully capsular serogroup B strain, MC58. The same strains were found in 14 healthy carriers in the general population of Bobo-Dioulasso (100% of ST-192 isolates tested for cnl). The presence of cnl meningococci that can escape serum killing and cause invasive disease is of concern for future vaccination strategies and should promote rigorous surveillance of cnl meningococcal disease.
在布基纳法索对急性细菌性脑膜炎进行强化监测期间，从3例患者的脑脊液样本中分离出了表型为NG:NT:NST的脑膜炎球菌菌株。这些菌株的ctrA基因呈阴性，但crgA基因呈阳性。分子分型显示，这些菌株携带荚膜缺失位点（cnl），属于多位点序列类型（ST）-192。PorA测序表明，所有菌株均为P1.18-11,42；P1.18,42-1；P1.18-11,42-1；P1.18-11,42-3；或P1.18-12,42-1。测序还表明，所有菌株的FetA受体基因均为阴性。血清杀菌试验表明这些菌株具有抗性，其抗性与完全荚膜B血清群菌株MC58相当。在博博迪乌拉索普通人群的l名健康携带者中也发现了相同的菌株（100%检测cnl的ST-192分离株）。能够逃避血清杀菌并导致侵袭性疾病的cnl脑膜炎球菌的存在，对未来的疫苗接种策略构成了担忧，应加强对cnl脑膜炎球菌疾病的严格监测。
